Sorry for the delay in reply ( first vacation in almost 2 years and was avoiding anything "work" related). At present we perform an antibody screen at the point of prenatal workup (usually 16-20 weeks). If the patient is Rh negative, we also perform an antibody screen when the patient presents with an order for RhIg (28 weeks). The question I was lookin for an answer to was the frequency we see a change from a negative antibody screen during the course of the pregnancy to a positive at term. From the replies there appears to be a very low occurrance of a change to positive, but the change appears to have a higher frequency of being a c antibody than anything else. Peter, thanks for the reference and the discussion on the article in Transfusion. As has been stated, this is an interesting topic!!