With Immucor, techs were used to a 4+ the vast majority of the time. With Biotest, you can expect a 3+ frequently. Sometimes, (much more frequently than with Immucor) you get a +/- or a 1+. Techs who are expecting a 4+ will shake too hard and too long and they can shake away a +/- or a 1+ until it looks negative. This has happened several times with us. For adults, it is a tollerable error (no IAT is done on adult patients). For newborns and cord bloods, we have always required IAT on negatives (weak D testing), even with Immucor. What we find with Biotest is that if it is weak at IS, it doesn't really get stronger at IAT, but it doesn't go away either. Our rule for Biotest is: if it is positive in any way at any phase, the person is to be considered Rh positive.