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Yanxia

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Everything posted by Yanxia

  1. Yanxia
    I post this question today, it is because I just met an ABpos baby,he is 3 months old, and still has anti-A in his blood, his mom is Bpos. Someone had transfused him with AB pos packed red cells yesterday, and I can still detect the anti-A in his plasma. I think it was stronger before transfution. So, the questions confused me are 1. B mom produce IgG anti-A , I used to think only O type produce IgG anti-A/B 2. 3 months the maternal antibodies are still here, so the half life of IgG is longer than 20 more days
  2. Yanxia
    I remember (not clearly) that AABB states for neonate less then 4 months, we should give type O washed red cells. If I remember rightly, but the IgG antibody' s half life is more than 20 days, then why we need 4 months to avoid the maternal sourced antibodies?
  3. Yanxia
    It seems like an acquired-B to me. Maybe you can try to lower the pH to 4.5 to see the lady's cells react with anti-B reagents.
  4. Yanxia
    The transfused cells are denser, so most of them at the bottom, and patients' own cells at the top.
  5. Yanxia
    I guess there are a mixture of alloantibodies anti-C and anti-hrs. What puzzled me is the autocontrol is neg, but the eluation result is pos. As for the eluation result, it maybe anti-LW.
  6. Yanxia
    Because the 37 degree C pos result was in tube, I thought maybe the room temperature saline reaction using tube will be better to interprete the result.Because card and tube are two different methods. Just personal opinion
  7. Yanxia
    Because of the normal B antigens on the cells, so we can see strong reaction on MF with anti-AB.
  8. Yanxia
    Brilliant post StevenB. I just out of curiosity, you said the after-adsorption plasma reactived with IAT, and had a titer of 16,384 , which is very high, but non reactive with PEG IAT. So interesting. If my understading of English is right
  9. Yanxia
    In my opinion, if we cannot select an antigen neg reverse cells, there are things we can do, it is adsorbing the plasma/serum using pooled O cells, then do the reverse typing, to get a neat anti-A or anti-B result.
  10. Yanxia
    Would you please explain why we can different the maternal blood and fetus's blood by using NaOH? Thanks!
  11. Yanxia
    Thanks for your advice. We often see neg DAT results with ABO HDFN in our work.( Because the A B antigens on the newborn red cells are weak ) You are right that I need an eluation result to support the HDFN . As to anti-A1, we got 3 pos with A cells and 3 neg results with O cells. And yes, maybe due to infection the baby's cells can be polyagglutinatable, just cannot interfere with the plasma reaction with donors and screen cells.
  12. Yanxia
    The baby's and his mom's plasma screen test is neg , and baby's plasma reacted with three different A donors' cells, so I suspect it is anti-A( anti-A1).
  13. Yanxia
    I have met two cases of babies. Their plasma had anti-A or anti-B, as their correspond type. And the doctors had not seen HDFN signs clinically. We found it through crossmatch.( we issue same type blood to infant have no ABO HDFN).
  14. Yanxia
    The baby's DAT is negative. Yes, the baby has anaemic and very bad sicked on the machine to help him breath. He has very servere infection, maybe this cover the symptoms which shows HDFN. And ABO HDFN is always mild. The jaundice is normal.
  15. Yanxia
    My experience and knowledge in bloodbank is so little. This is the first time I met an warm reactive anti-A1 and which can pass the placenta barria. It is new to me.
  16. Yanxia
    Sorry , I cannot sure about that. Is there IgG anti-A1 exist in subgroup people?
  17. Yanxia
    I just encounter a 3 days old baby, his type is A pos, and has anti-A in his blood. His mom is A subgroup B. If the baby have anti-A, it is IgG. Is it possible an A sub B produce IgG anti-A? Thanks for your advise.
  18. Yanxia
    If the reverse reaction with B cell was a typo, I agree with Malcolm, it is an anti-A1or anti-ALeb. As for the negative saline replacement result, since the neat plasma reaction is weak, it maybe weaken by the test method.
  19. Yanxia
    If the patient has received transfusion, the transfused antigens pos cells can cause the autocontrol mixed field positive, and when the antibodies are against some low prevalence antigens, then the reaction with screening cells and donor cells can get a neg reaction. Or some drug induced antibodies can cause this kind of reaction because they are drug dependent.
  20. Yanxia
    Sorry, I am stubborn as for this major, I guess I have caused noise here. If the reverse type show antibody then it is ok, why would we call 2+ or more to be normal and less to be weak and then to invest it? For ABweak patients, I still think it is safe to transfuse them with AB plasma, even they have their anti-B ,but the anti-B is not the same as O and A people's, it is not react with its own B antigens, but the transfused anti-B can, that is why the weak B antigen can be detected with some strengthen method. We will identify this kind of weak antigen with add more serum ,4 degree C incubation or adsorption/elution test, not genotype it, which is not so expensive.
  21. Yanxia
    I totally agree with you about the transfusion, galvania. And I think we should call it AB subgroup if there are weak B antigens, rather than A group, because sometimes they will need plasma and something contains plasma.
  22. Yanxia
    In the case of ABel, the forward typing is not shown B antigens, and reverse typing with weaken anti-B.
  23. Yanxia
    This is my understanding, maybe it is not correct, just want to share it here, and correction is always welcomed. If the reverse typing is weak than 2+, it means the immediate spin result, the antiboies are weaker than normal, subgroup or weaken antiglobulin. Even we incubate or change the reaction temperature or add more serum, it shows 2+ or stronger reaction, it is still weaker than normal typing( not so exactly, because my Enligh is not good). Can we call it normal when we incubate or 4 degree C treaction 2+, no. These are just ways to strengthen the reaction. Which is somehow like we do weak D test, can we call it normal D when we get pos result in anti-antiglobulin test.
  24. Yanxia
    I agree with you if transfusing RBCs, on the other hand, plasma transfusion, I think it is safe to test if the weak B antigens exist or not.
  25. Yanxia
    I would add more antisera and/ or incubate the forward test at 4 degree C to see if there are B antigens here, if negative then adsorption and elution on the forward B antigens to see if it is existed. The most important thing as Malcolm mentioned above keep a group O cell as negative control. If all the above tests are all neg, then I will call it A type with weaken anti-B.
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