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LabLion's Achievements

  1. Did you ever figure out the validation protocol for washing platelets? If yes, would you please share the information? I am in the process of validating. Thanks
  2. I would appreciate any help from users of HMS Plus. what is you protocol for linearity? Do you use Hepline Kit.... Thanks Lablion
  3. Thanks all for the good discussion. I would like to point that STRAC is NOT a transfusion related service, and their data is primarily based on outcomes of ambulance and air care patients. How many of such patients have hemolysis, and morbidity related to all things Dr. Blumberg points out, is neither studied OR reported properly. Secondly, I believe (I may be wrong that STRAC is involved in and researches whole blood transfusion in trauma settings. Whereas we are talking about using whole blood in in-hospital and L&D settings which it totally different and uninvestigated. Hence, I used the words "extrapolation" and "assumptions" at the begining of this thread. What I meant is, STRAC is extrapolation data from Military, and Hospitals are trying to extrapolate from data from Ambulances and fire fighters. Lablion
  4. Thank you Ward_X, SMiller and Dr. Blumberg for your time and reply. I Agree with the above: There is so much extrapolation and assumptions involved in the decision to use Low titer O group in Hospital settings. Like for e.g. the proponents are showing military data as evidence, but the military uses WARM Fresh whole blood. Secondly, data is about penetrative trauma and whole blood is giving at site or within the golden hour. Urban hospital settings are so much different. Third, the proponents claim success of programs at Utah, Pittsburg, Mayo etc. However, so many questions (see below) are unexplored or unanswered. I agree with SMILLER about practicality of use. If one were to set up Low titer whole blood: 1). In which scenarios or patients would you use? (trauma or other massive bleed?, penetrative trauma vs blunt trauma, is there a time limit within which it has the best beneficial effect? What sex and age group to use for? 2) What titer is acceptable? IgM and IgG, whether the titers are done in viv from Patients draw or from the unit, whether the titer is historical or done every time the donor donates? 3) what anticoagulant is used for storage....whether titers should be different for different volumes of anticoagulant (dilutional effects?) 4). Leukoreduction? 5). Is old whole blood (>14 days) as beneficial as fresh cold whole blood (1-14 days) or as WARM whole blood? Too many questions But the most significant if wish to explore is, How to set up hospital criteria to issue whole blood? Thanks again for you insights and time. Lablion
  5. The only previous listing on this topic was by SMILLER in 2017. I wonder NOW has anyone moved to using STORED whole blood in a tertiary care urban hospital. The Story so far: Our supplier has an inventory of whole blood (some of which is used by the Fire dept or first responders at site of trauma). They would like the hospitals to use whole blood for massive transfusions and are trying to convince the surgeons about the advantages. Question is: What are the advantages (if any). What are the disadvantages. What would be the indication to use whole blood (instead of the massive transfusion protocol that we currently use). What about the logistics of matching blood types? (also I know most whole blood are obviously not leuko reduced). I realize there are many questions, but I appreciate your time and any response. Thanks, P.S. I just saw Jayinsat write updates about the conference in san antonio in 2019. I read the online presentions, but still didn't get convince about in hospital use of the product. Lablion
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