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comment_71829

Hi Everyone,

Does anyone have a validation protocol for washing platelets in the Cobe 2991 Cell Processor?  Any guidance is much appreciated!

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  • Neil Blumberg
    Neil Blumberg

    All you need to do is document platelet recovery (should be at least 80%) and pH.  Nothing else is required.  No platelet function tests, etc. since these are not used with unwashed platelets.  Would

comment_81339

Did you ever figure out the validation protocol for washing platelets?  If yes, would you please share the information? I am in the process of validating.

Thanks

comment_81342

All you need to do is document platelet recovery (should be at least 80%) and pH.  Nothing else is required.  No platelet function tests, etc. since these are not used with unwashed platelets.  Would recommend washing in platelet additive solution rather than saline.Washed platelet buffy coat platelet additive manual.pdf

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