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    • By Lucas Souza
      Hi, guys 
      How are you?
      So, i'm doing a experiment where i'm testing two titration methods, the tube test and the column agglutination technique.
      Now i have to analyze to know if they have statistical difference and what is the correlacion of the two methods, but I found it difficult to decide which statistical test to use.
      Do you have any thoughts on which statistical test I should use? T teste? Pearson?
      Below is the table with the samples and their endpoints, for example:
      PLASMA TUBE A GEL A  TUBE B GEL B 1 128 256 32 64 2 32 32 64 64 3 64 64 32 16 4 64 32 32 16 5 64 32 64 32 6 32 32 32 16 7 64 32 64 32 8 32 32 32 32 9 32 32 32 16 10 64 32 32 32 11 64 32 64 16 12 128 64 128 64 13 64 32 64 32 14 32 16 32 16 15 64 32 128 64 16 128 64 32 16 17 128 64 64 32 18 64 64 16 8 19 64 64 64 32 20 32 16 32 16  
      Thank you guys.
    • By WMMTSBB
      I had a patient that had an ABO discrepancy. Testing is as follows:
      anti-A: 4+, anti-B: 4+, A1 cell: 1+s, B cell: 4+, Screen cells I.S.: all negative, LISS 37: all negative, poly AHG: all negative. Group O donors I.S.: negative, Group A donors: 1+ to 2+. Saline replacement for A1 and group A donors: negative. Microscopically, the A1 and A donors do not really appear as rouleaux. So the question is why do we see apparent rouleaux only with the A cells and not the screening cells?
    • By mpmiola
      When nonavailable ABO-identical platelets. What to transfuse?
      Example:
      Patients of group A
      1st choice: AB (incompatibility major) 
      2nd choice: B (incompatibility major) 
      or
      1st choice: O (incompatibility minor)
       
    • By aafrin
      We had a 46 year old male health check patient who has never been transfused before come for blood grouping. He knows his blood group previously as O Rh Positive. Our results were as follows:
       
       
      Tube Test
      BioRad Gel
      -A
      1-2+ mf
      0
      -B
      0
      0
      -AB
      2+mf
      ND
      -D (igG+IgM)
      4+
      ND
      -D (IgM)
      4+
      4+
      A1 Lectin
      0
      ND
      H Lectin
      4+
      ND
      A1 Cells
      1-2+ at RT, >2+ at 37 C
      w-1+ at RT & 37C
      B Cells
      4+
      4+
      O Cells
      0
      ND
      Autocontrol
      0
      ND
      A2 Cells
      0
      ND
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
      We repeated the blood group with two other manufacturer’s anti-sera. One anti-sera gave the same result as our tube test result, but with the other anti-sera the result with anti-A was negative.
      What should we report the blood group as? O or subgroup of A? Kindly help.
    • By Jermin
      Hi,
      This is a very silly question, but I think I have confused myself. Why is it that when Rh phenotyping, we do not require incubation step, but when looking for antibodies, we need incubation to detect Rh antibodies? 
       
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