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exlimey

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Everything posted by exlimey

  1. exlimey
    I agree with Malcolm's comments and would add that I suspect that many positive DATs in donors occur after collection of the blood, i.e., the donor is not actually DAT+ in vivo, but somehow the red cells pick up globulins during exposure to the chemicals and environment of the collection system.
  2. exlimey
    sbazel - bad idea to use the earliest expiration date of the components of your cocktail. Neither apply to your working reagent. Certainly the anti-D has been grossly modified from its original. To assign a genuine expiration date to your cocktail, you would be obliged to perform a formal stability protocol.
  3. exlimey
    If you are using a commercial anti-D reagent as your starting material, you might be creating your own problems. Most reagents are now monoclonal and as such they often have very peculiar formulations (additives, diluents, etc.) that ensure their stability. When end users dilute or otherwise modify the reagents, they may lose the key element required for reactivity and/or stability (even when frozen). I suggest you try a polyclonal (human) source (which is probably what Malcolm's group is using). If you need to dilute it to get to the desired reactivity, I recommend using either inert normal human serum or 6% BSA. Both diluents should maximize your chances of stability, either in the liquid state or frozen.
  4. exlimey
    Winter - in the case of DARA patients, the DTT is use to treat (panel or screening) cells rather than the serum. SMILLER - do you enzyme-treat your own cells ? If so, have you determined their stability ?
  5. exlimey
    You will probably need to "qualify" the vendor is some fashion, but I don't believe you have to do any serological comparisons providing you are going to use the material according to the manufacturer's directions.
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