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Dansket

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Everything posted by Dansket

  1. Dansket
    Thanks again!!
  2. Dansket
    Thanks for your input BankerGirl. What is the size of the MobiLab label, is it smaller or larger than your standard Meditech label?
  3. Dansket
    BankerGirl, My BBK Label format is highly modified for our purposes, e.g., these items are printed only on the BBK label: Historical Blood Type, antibodies identified, newborn's time of birth, patient's mri number barcode. Will I lose these? Dan
  4. Dansket
    Does MobiLab use the individualized module (LAB, MICRO and BBK) labels as defined in the Label Format dictionary or a single generalized format for all modules in Meditech?
  5. Dansket
    We had a custom report created by Iatrics for Meditech C/S 5.6x that prints the form that we attach to the unit for emergency release. The program requires only 3 inputs, patient name, unit number and MD name. This gets the RN out of blood bank as quickly as possible. Then we made a second entry using the Meditech Emergency Release routine at our leisure.
  6. Dansket
    Validated transport coolers for storage up to 24 hours. Standard blood bank refrigerator with remote alarms. Helmer has mobile blood bank refrigerator. HemoSafe blood storage/issue refrigerator. These are some of your options.
  7. Dansket
    My bad, I intended to state, "If the Weak D test is positive on a newborn, we report the patient to be Rh Positive, if the Weak D test is negative, we report the patient to be Rh Negative.
  8. Dansket
    If the Weak D test is positive on a newborn, we report the patient to be Rh Positive, if the Weak D test is negative, we report the patient to be Rh Positive.
  9. Dansket
    If using a paper results entry system, then I would the same methodology for immediate-spin crossmatch as the ABO plasma grouping test. If using a computer results entry system (properly configured), I would enter initial immediate-spin crossmatch results obtained without enhancement.. A properly configured computer system will not allow crossmatch of ABO incompatible rbc unit (regardless of whether or not electronic crossmatch is available)! In my world, your patient would be electronically crossmatched after all required pretransfusion compatibility testing requirements had been met. We see such patients routinely where the expected anti-A and/or anti-B is not detected in ABO Plasma grouping test done in gel. To enhance detectability of the missing antibody, we do immediate-spin tube test with 3% A1 and/or B cells which resolves 80% of this type of ABO grouping discrepancy.
  10. Dansket
    Does this mean that a physical specimen is retrieved from storage?
  11. Dansket
    Are you sure? A test name, a lot number and an expiration date is not much different than a test result!
  12. Dansket
    amym1586, Depending on your BBK computer system, you can create a polydat test that cannot be resulted without QC (make the QC results entry part of the test results entry).
  13. Dansket
    Frenchie, Is there an Order/Entry interface between your HIS and the Meditech BBK module or are all BBK tests manually ordered in Meditech?
  14. Dansket
    Yes, quite right. ProVue uses manufactured reagent red blood cells for reverse grouping, antibody screen and antibody identification. However, fresh cell suspension are made from each test sample.
  15. Dansket
    Another advantage of automated testing, all cell suspensions are freshly made.
  16. Dansket
    I never stocked polyspecific reagent, just anti-IgG and anti-complement reagents separately. Test adult cells with both if physician requested DAT on adult and when working-up patients with autoantibody.
  17. Dansket
    If the standard serial dilution process produced a 1+ or stronger result in the 1: 256 dilution tube, I would make a master dilution of 1:500 using a 50mL volumetric flask. Subsequent dilution would be 1:1000, 1:2000, 1:4000, etc.
  18. Dansket
    It would be incredibly important to scrutinize the LR filter's manufacturer's direction insert to verify that the LR filter can be used for platelet components.
  19. Dansket
    I agree with Michael when you add 50ul saline to 50ul plasma and 50ul cells your concentration is different then what is in package insert. I am sure you must have validated your procedure as you are adding saline and technically diluting your plasma. when we do saline replacement in tube, we only use it to resolve discrepancy not to ID antibody! DO you take same tube where you perform saline replacement and carry through all phases... My original post was in response to StevenB's post regarding to his perceived limitation of gel testing. Regardless if the plasma is technically diluted 1:1, if it resolves the ABO discrepancy, I run with it! In the past, I limited the use of the saline-replacement technique in standard tube testing to resolving ABO discrepancies detected in the immediate-spin ABO plasma grouping test. I have not used saline-replacement for antibody identification.
  20. Dansket
    OK, point well taken. If you add 50uL of low-ionic strength saline to the mixture of 50uL plasma and 50uL 0.8% rbcs, then the ionic strength as well as the plasma/cell ratio is maintained in the reaction chamber This provides a tool to mitigate rouleaux formation in Gel.
  21. Dansket
    Me, either. Forget the math! The total volume in the reaction chamber is 150uL. The number of antibody molecules present is the same, despite the addition of saline to the mixture. I don't see this as any different than what is done in test tubes when 1-2 drops of albumin or a LISS additive solution are added to one drop of 3% cells and 2 drops of patient plasma. What changes the serum/cell ratio is adding four (4) drops of serum to one (1) drop of cells in an attempt to enhance the reactivity of a weakly or non-reactive anti-A or anti-B in an ABO plasma grouping test.
  22. Dansket
    Malcolm, I'm not diluting the plasma prior to adding it to the reaction chamber. For example, ABO plasma grouping test requires 50uL of plasma and 50uL of RRBC. If you add 50uL of 0.8% RRBC, 50uL of test plasma and 50uL of saline, the ratio of plasma to rbc does not change. Whether or not saline is added, there is 50uL of plasma and 50uL of 0.8% RRBC in the reaction chamber.
  23. Dansket
    Granted you can't do 'saline-replacement' in Gel, but you can add additional saline to the gel card reaction chamber!
  24. Dansket
    I would stay away from Thermo Scientific aka Jewett. I prefer Helmer's magnetic door closure.
  25. Dansket
    We follow the same process for antibody identification whether it is being done the first time or the 21st time on a patient.
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