mrkeramati

with buying the new screen cells or changing in lot numbers of vials of antibody detection in the blood bank, we need to evaluate them for appropriate quality. how do you perform this?

When we encounter with this Rh blood group how to report it and what is you advise for kind of Rh if the patients need blood transfusion?

we do not use FDA licensed reagents and some time we have encountered with some problems using the reagents.

I would like to know the practical methods with details for QC of anti-human globulin, anti- A, Anti-B and Anti-D reagents after buying a new trade mark or change in lot numbers.

We measure PT and PTT tests with using sysmex (CA50) or Coalaser (DiaLAB) analysers using Biolab reagents. Both instruments measure the tests according turbidometric method. We encounter a "NC error" (PT>60 sec and PTT>180 sec) for about each 20 tests in non turbid patient's plasma. However, when we measure these samples with manual methods or by other instrument even with turbidometric method ( ACL analyser) and using the same reagents, the test results is normal/ mild to moderate increase. How could resolve this problem?

i would like a guideline for applying quality management system in a blood bank. would you mind helping me to apply it?

We often observed some patients have ABO subgroups such as A3, Am, A int, Ax, B3 and so on in blood banking. Using some methods such as adding Anti-H, Anti-A1 or Anti-AB to patients RBCs somewhat helps us to make this differentiation. However, I would like a way to differentiate these subgroups step by step so that we can use it in a hospital blood bank.

yes. if it is possible

Dear ahmed, I was wondering if you could send me your protocol with details for me. Thanks

Dear mlam, thanks for your reply. I have a few question. 1. We dont have monoclonal Anti-D containing only IgG antibody. Is it possible to prepare check cells with Anti- D containing IgG+IgM? 2. are you use check cells from packed RBC prepared from the donor blood bags or blood prepared from the patints? if you use the last one do you add preservative to this aliquot. 3. Why you use from high protein D reagent? Thanks,

I want to prepare check cells ( coomb's control cells). I need a protocol for it. woul you mind sending it to me.

We stain peripheral blood smears with May-Grunwald-Geimsa. But some smears do not properly maintain staining properties during long time maintaining. Especially after about two years, they loss basophilia of nucleus and we only see a hollow space in location of nucleus; however, cytoplasm maintain its staining characteristics for longer. Indeed, this occurrence does not happen for all slides. We also have slides stained more than ten years ago but despite it they have good staining characteristics