lacs

Phew! I was worried! I never saw it the last time I renewed. I am good then. Thanks ALL. You guys are great!

Thanks ALL for the reply. What do you do about getting credits for the following areas? a)Research and Preparation for Presentation Authorizing a book or book chapter, doctoral dissertation c) Editing a book d) Presenting posters/exhibits e) Journal articles, master thesis f) Serving on examination committees, committes or boards related to the profession g) Role of on-site inspector/paper reviewer for laboratory accreditation (JCAHO, CAP, etc,) or training program accreditation (NAACLS, CAAHEP) 2pts/year. I don't know about you but I don't have opportunity or able to participate in all of them. Help!!! Aren't we supposed to full-fill all of them??

Does anyone know if we are required to participate in the CMP program if you're an SBB, or is it really optional?

2 homos to rule in and out.

We normally wait for 24hr of continuous monitoring before using it again. Rarely, we use 4hr time-frame.

We do one from for a batch (for example, if they request 2) and we write the unit # on the form.

Somewhat related topic, does your SOP tell you to test for endpoint when adsorb using auto cells? If not, how do you know when to stop? How long do you incubate if you test your plasma against DTT-treated red cells in tube? I want to say 30min (similar to saline) since there is no enhancement but I've not been able to find literature to say exact time.

Unless our patient is also a sickler, we still adsorb. We do PEG adsorption. It's only because we want to save phenotype matched units for those who truly need them, due to shortage of good red cells.

We run 3 screens-one w/o enhancement at IS, 37 and Coombs, PEG and GEL. Our problem is when it only reacts in GEL and looks like HTLAs. We are limited to what is tested. We do ficin, cord in gel and rarely DTT or titer since titration result is typically higher in gel than tubes. How are you all handling reactions in GEL only?

That sounds logical! Thanks you all for responding.

Thanks for the info. Hmm let me double check the PIs they sent us. I am almost 100% certain that it requires unbuffered saline for antisera. No, we're not in Canada. We're in the US.

We are considering purchasing the partial D and competency kits as well. Our local hospitals do not seem to like their ABO/Rh kits. Reactions come off "sticky". Also, we are using buffered saline and their antisera requires unbuffered saline. How are you guys handling that, if you currently are using buffered saline cubes?

Since we're on the same subject. We just had a female patient (much older than a typical childbearing age) who has anti-D and C in her plasma but we were able to dilute anti-D and C in eluate as well. We ruled out passive antibodies. Is it a stretch to think the she received rG at some point and attaching to rG cells from current transfusion? This is still a rare situation. It's almost always an academic interest for us so we hardly work up samples like this, unless there is a good reason to do so, due to costs. What else could it be?

Do you report of cold antibody based on 4C testing only? AKA, no other reactions at IS or RT.

One more question, Our Fy(a-b-) cells came up with ficin. That's odd. Fy3 is resistant to ficin but it should not come up if it's not there to begin with. Any thoughts. One another subject, if you have a typical WAA but is reacting at IS and 37 and RT and 4C, do you call it WAA broad thermal amplitude or mix of cold and warm?