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Shelby56

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Everything posted by Shelby56

  1. Our blood supplier provides low titer (<200) leukocytes-reduced whole blood.
  2. We have multiple Visions, our cords are run on a separate instrument- I have spoken to Ortho regarding this and they do not support testing without specific QC for the testing being performed. Most institutions use vial #4 with AlbaQ-Chek to prepare positive DAT samples. Happy New Year to all.
  3. Well- I'm not sure who told you that, but you have to have some QC for testing done that doesn't have commercial QC material. Thank you.
  4. Hi- we are updating our procedures to reflect manipulating AlbaQChek QC to create a positive DAT. Does anyone know is this just an "internal" QC thing or IQCP? Sorry if this seems like a dumb Q...I'm adding to Vision QC but didn't know how strict the monitoring has to be. Thank you!
  5. Hahaha this is a great group- na just good old anti-D that is 4+ and would cling or carryover on the probe
  6. We are using a "known" high titer (4+) commercial antisera- run in line with a known negative plasma....after pipetting the 4+, the known negative is expected to be negative.
  7. Hi- we are a 360 bed T level 2, we have dedicated Blood Bank staff on first and second shift and 3 FT BB rotational staff. It is imperative for us- even with the continuity, it's still an issue to know all the needs of each recurring pt...competency biggest issue - glad not numerous generalists.
  8. Good morning- I have always followed the CLIA / CMS / CFR guidelines, which requires us to follow the manufacturer's instructions. When you read the inserts, the reagents we are using clearly state do not use beyond expiration. I don't consider expired panel cells to be acceptable, as many QC for reactivity, but don't test for each antigen trying to include or exclude. It is an old practice, the same as not QCing upon receipt, and noting reactivity of any kind to show acceptable performance. We have not had any additional expense nor identification issues with using in dated reagents in our Transfusion Service. I have trouble not citing folks when I see old outdated panels filling banks on inspections.
  9. We also send out WAA work-ups to our reference lab. The competency and proficiency committments are also very labor/staff intensive to cover all 3 shifts. If we have one tech covering the Blood Bank and 1 or 2 trauma codes come in such as GSW, there is no way the only tech could spend hours adsorbing one patient's plasma / cells. Also, for allogeneic adsorptions, you have to have a supply of cells for that too readily available. Not expense driven for us to send these out.
  10. We also had to change from the 30 minute rule to taking the temps of RBCs and also had to establish a 1-6 C for returned (considered storage) vs 1- 10 C if "in transport". All of the returns which are not in coolers are out of temp when returned within 30 mins and we cannot accept Apheresis Plts at all due to documentation issues regarding their storage while out of our control for periods of time, esp the OR which tends to place items in coolers even with the "room temperature" stickers on them lol. Would love to see more studies regarding the 60 minute return would save alot on wastage.
  11. We also had this problem with February's standing order of 0.8% screening cells - SCI reacting 1+ and two cells on Panel A that also happened to be pos for K (Kk) same 1+ reaction. Luckily the Panel B we had available had one cell that was KK and those same patients tested negative with that homozygous cell. I did report to Ortho J&J because was weird the SCI was Bgb neg while the panel cells were both Bgb positive. Really don't want that donor in future lol
  12. Yes- we do that also- for each daily-but not by the reagent QC- ty for your comment.
  13. Sorry- I miss the logic ...if you are performing reagent QC and use the exact same gel cards and reagents (such as screening cells) whether sitting with it in front of you, or allowing the ProVue to do it-what are you going to find by performing duplicate QC? We have several centrifuges and incubators, if only one set is used for QC-what about all the others that are used during the day? That is instrument QC and is done separately. We used to do both too, but have now stopped the practice because it wasn't necessary-so this string threw me off a little.
  14. Hi all-I have a related question but not titers- do you perform AHG crossmatching for these patients if need transfusion until anti-D no longer present (if passive)? We have a big debate here about that....
  15. AABB standards refer to using either the maternal or neonatal plasma - you can test neonate, then give units that either do not contain the corresponding antigen OR are compatible by AHG xm.
  16. A hospital in our area called today to share a tale about a patient who typed as A Rh pos on the ProVue w/ no plasma /cell discrepancy in ABO gel card, but was an A subgroup (lectin negative) with 2+ anti-A1 in tube testing. The crossmatches with A1 RBCs were compatible on ProVue, but incompatible in tube testing. Has anyone else ever seen this and what kind of clinical complications would be expected if RBCs ( A1 w/ anti A1) are transfused? Also, what are some opinions if the ProVue can't detect weaker forms of plasma /cell discrepancy-really a challenge if only using ProVue and computer crossmatching. An Ortho resource says it has to do with "harder spin in tube centrifuge v softer spin gel centrifuge". Thanks!
  17. Our pink and purple tubes are both the same size ( 6 mL ) but- we switched to the pink top for the BDID PIK (patient identification check) because it is notched and has a barcode on it-both K2 EDTA 10.8 mg
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