[New Ortho vision user]

Hi we are currently in the process of implementing ortho vision analysers across our health board in the UK. I was wondering if anyone who is currently a user of the instrument would mind sharing their SOP with me? 

Many Thanks. 

[Techniques for detecting cold antibodies]

If you have a grouping anomaly which is suspected to be due to a cold reacting antibody, do folk set up saline cards with Coombs panel at room temp and /or 4c to help identify the antibody? 

[False Positive KB test]

We in our blood bank have sent away kleihauer positive samples for flow cytometry testing which has come back as negative as the technique looks for 'RhD Antigens' on the foetal red cells, in which case it is likely that the foetal cells are D negative and a negative flow result is returned. 

[Project ideas]

Hi Malcolm...it is a district general hospital blood bank, uses Diamed technology. 

[Project ideas]

Hi all,

 

i have a colleague who is looking for ideas for his BSc biomedical science honours project, does anyone have any ideas? 

 

Thanks

 

Tricia 

[HSD]

Hi gagpinks , i successfully passed the HSD in transfusion in 2010, so if i can be of any help feel free to get in touch!

Currently waiting on my exams results from the HSD in laboratory leadership and management which are released on Friday --eekkk!

 

Thanks

 

Tricia

[Anti-D quantitation]

That is brilliant, thanks very much Malcolm 

 

kind regards

 

Tricia 

[Anti-D quantitation]

Aw that's great thanks Malcolm, sounds like a super weekend! Enjoy! 

 

 

[Anti-D quantitation]

Hi,

 

Could anyone please describe to me the basic principle of the Autoanalyser for measuring anti-D levels please

 

Thanks

 

Tricia

[Antibody interfering with blood grouping]

likewise Malcolm, always nice to put a face to a name 

[Antibody interfering with blood grouping]

Thanks for the responses

Hi Malcolm, not sure if you remember but I spoke to you at Scotblood this year!

 

tricia

[Antibody interfering with blood grouping]

Hi , we have in the last couple of weeks had two cases of patients with anti-c , reacting with the A cells of the blood group, i have never saw this before ( only the usual cold reacting antibodies such as Anti-M etc)  and was just wondering if this is something that others experience and why this is happening? The supplier has confirmed that the A cells are A neg (rr) cells. 

 

 

Thanks

Tricia 

[NIBSC]

Hi,

 

does anyone use a NIBSC anti-D on their blood bank automation...to demonstrate metrological traecability...? 

 

Thanks 

[Positive control with crossmatch]

Hi ,

Does anyone routinely run a positive control with cross matches on analysers? 

The BCSH guidelines state that a positive control should be included with each cross match -and we do this for manual techniques but not on the automated technique....grateful for thoughts on this...

thanks

[QC for blood bank analysers]

Hi all,

 

I was just looking for people's thoughts on the use of an AB negative, antibody screen negative QC material for controlling blood bank automation. Apparently this was a recent UKAS finding that this wasn't been used. We currently use BIORAD basic QC 1 (A Neg - Anti-D ) and QC2 (B pos Anti -Fy(a) ) . Does anyone use an AB Neg/ negative QC routinely, and was looking on thoughts on why this is required over and above the two we already use? PS wasn't our UKAS inspection, but we have one pending!

 

many thanks

 

 

[Uncertainty of measurement]

Hi 

 

would like to know how labs calculate uncertainty of measurement for quantitative methods ie fbc, coagulation tests, and how they calculate network uncertainty across their shared sites. 

 

Thanks! 

[Auto Anti-D]

Hi Malcolm as far as I am aware it is a proven auto anti-D , but units they are providing are least incompatible , yet rhesus neg units are compatible, I will look into it in more detail tomorrow and speak to reference centre, will post an update....

thanks

[Auto Anti-D]

Sorry PVP, patient versus plasma auto control.

[Auto Anti-D]

Hi,

 

I was just looking for advice/ clarification on the units which are given to someone exhibiting an auto anti-D. The patient has had recent transfusions and the blood which comes from the reference centre is rhesus positive, least incompatible units. However when we cross-match rhesus neg units for the patient in the laboratory they are compatible. I was just wondering why this was. I looked at the guidelines but I'm not sure they answer this question. I was only briefly made aware of this case yesterday so there may be more to it, but his panel only displays an anti-D. PVP positive. 

 

Thanks 

 

Tricia 

[Re:help]

Thanks Malcolm, TRALI, I think is where I might go as I know we have had a couple of suspected cases and the staff would benefit from expanding their knowledge on the subject, thus if anyone has experienced any interesting cases in their own labs I would be very grateful if they would like to share this info.

Many thanks

Tricia

[Re:help]

Hi all,

I have been asked to do a lunchtime presentation to my fellow colleagues in the haem/blood transfusion dept of a large district general hospital. I was wondering if anyone had any interesting topic suggestions. I like to present topics where I can discuss real life scenarios which have happened in other transfusion depts both in UK and across the pond ! and this site has been very helpful in the past in providing such info.

Any suggestions would be much appreciated.

HDN is being covered by a case study by someone else.

Many thanks

Tricia

[UKAS inspection]

Hi we are due to have a UKAS surveillance visit, and I believe one of the points that have been raised in other sites is regarding the use of a national standard for anti-D. Apparently after a PMI on an analyser we should be re validating the analyser with a primary standard of Anti-D. The basic QC which we use and which contains an anti-D of 0.05iu/ml is traceable back to a WHO standard.

Any thoughts?

Thanks

[help with ABO grouping anomalies]

hi yes it is sorry

[Blood grouping anomalies]

Hi there, I was hoping that someone could help me out with the following grouping anomalies and what their interpretation is.

 

1)what group would this be - reaction with anti-A -4+, Anti-B - neg, PVP-neg, A1 cells - 2+, B cells 4+ and O cells - 2+

 

2)what causes this?  reaction with anti-A - 4+, Anti-B -1+, PVP-neg, A cells- neg, B cells-4+ and O cells-neg.

 

thanks

 

Tricia

[help with ABO grouping anomalies]

Hi there, I was hoping that someone could help me out with the following grouping anomalies and what their interpretation is.

 

1)what group would this be - reaction with anti-A -4+, Anti-B - neg, PVP-neg, A1 cells - 2+, B cells 4+ and O cells - 2+

 

2)what causes this?  reaction with anti-A - 4+, Anti-B -1+, PVP-neg, A cells- neg, B cells-4+ and O cells-neg.

 

thanks

 

Tricia