[neonatal transfusion]

Our policy is to issue O Group PRCs and group AB FFPs to a neonate (<4 months age). The Rh of PRCs will depend on the Rh status of the neonate. Top-up PRC transfusions are usually from one single unit <28 days.

[Biorad IH 1000?]

We were using IH 1000 in 2015, but there were too many problems in their software, due to which it would discard lot of cards. Hence we returned it. Maybe they have improved the software now.

Also, I've heard they have another smaller version IH 500 released a year or so ago.

[Help with ABO Group]

On 3/30/2018 at 6:54 PM, mpmiola said:

1. Are the reagents you used from different manufacturers from the same clone? Do they have the same title? Differences in clone or titer may lead to differences in reactivity.

They are from different clones and titer.

We repeated the blood group using BioRad Newborn Card and it showed 2+ with anti-A,B but negative with anti-A.

[Help with ABO Group]

We had a 46 year old male health check patient who has never been transfused before come for blood grouping. He knows his blood group previously as O Rh Positive. Our results were as follows:

	Tube Test	BioRad Gel
-A	1-2+ mf	0
-B	0	0
-AB	2+mf	ND
-D (igG+IgM)	4+	ND
-D (IgM)	4+	4+
A1 Lectin	0	ND
H Lectin	4+	ND
A1 Cells	1-2+ at RT, >2+ at 37 C	w-1+ at RT & 37C
B Cells	4+	4+
O Cells	0	ND
Autocontrol	0	ND
A2 Cells	0	ND

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

We repeated the blood group with two other manufacturer’s anti-sera. One anti-sera gave the same result as our tube test result, but with the other anti-sera the result with anti-A was negative.

What should we report the blood group as? O or subgroup of A? Kindly help.

[Polyspecific]

We do the same as AMcCord and Mollyredone.

[Use of wash RBC´s]

We use washed RBCs for intrauterine transfusions. They are very few and far between...

[Do you use microscope in transfusion services?]

We use microscope, if we suspect mixed field, rouleaux or weak/barely positive reactions seen macroscopically. We generally use gel for DAT, etc, so no need for microscope.

[Expiry date and time of thawed FFP]

In the UK some labs are using the 5 day rule.

But that will be as plasma not FFP.

[Expiry date and time of thawed FFP]

Usually thawed FFP has expiry date of 24 hours if stored at 2-6 C, else 4 hours at RT.

[HDN procedures]

We do front type and DAT on all cord blood samples as we use the BIORAD gel test, but we don't report DAT result for all. pediatricians usually ask for DAT if mother is Rh neg. or has known antibodies.

We have never done immune anti-A or -B. test  We do elution if pos.DAT cannot be explained by mothers type and/or baby will require transfusion.

[How long do you hold units in crossmatched status?]

We usually keep x-matched units for a day further from the day on which it was requested. Rest as Terri, and YES we do x-match units to multiple patients - First in First out (FIFO) to avoid outdate

[Unlabeled specimens]

Such a policy will endanger lives of patients. Unlabelled samples should never be processed, nor should they be allowed to be labeled in lab by even the person who drew the sample. Safety first principle is paramount.Such samples should be dumped and new sample properly labeled in presence of patient should be collected.

[TRM.31450 Comparability of Instrument/Method - new checklist 7/11/11]

Here's my version. It's pretty simple, but it passed a CAP inspection last month. [ATTACH]527[/ATTACH]

[ATTACH]528[/ATTACH]

I cannot open this attachment.

 

I would also appreciate if anyone could share their SOP or worksheet for comparison of methods.

Email- aafrin@gmail.com

[Time limit for type and screens on outpatients]

@CSWICKARD Could the plts & FFP transfused be stained with RBC's ? Just a thought...

 

[How do you report a titer result?]

We too report it as 64.

[Cord Blood Testing on the ProVue]

We also use check cells as our Positive QC for gel. We do not have Provue.

[Least Incompatible Form]

None of the attachments can be opened. Phil please help?

[CORD DAT post RAADP]

Are both the anti-Ds "DVI-" in the card you are using?

Again - how do you interpret when one well shows neg reaction and other positive?

[Incidence of ABO HDF/N in Newborns of Non-Group O Mothers]

Not to be a whippersnapper, but I have also never seen a case of ABO HDFN in a non-O mom in 24 years.

[Charging for antigen typings]

I agree with Goodchild. We also charge both patients.

["Legal" release blood]

Yes but we call it "best matched".

[Loss of B Antigen]

Malcolm, we have a premie born in Oct.2014 who was originally A positive, but due to number of O positive red cell transfusions (top-up) now types as O positive. I have a question:  Should we continue to transfuse O positive red cells even after baby completes 4 months age or change to the original A group as the neonatal period comes to an end?

[QC for MTS IgG cards]

We do the same as Sandy L.

[A Christmas Story]

Merry Christmas & Happy New Year to all our PAthlab friends.

May the year 2015 usher in peace, love and goodwill all over the world.

God Bless!

[Manual Entry and 2nd tech review?]

We also use tube testing for Blood Grouping and MAnual gel for crossmatching. We do a lot of blood groups daily and all the groups are read by two techs independently who sign in the register. The same tubes are then reviewed by me or MO for the blood bank and we also sign in the register.To avoid mix-ups when dealing with multiple patients, we number each patient sample tube with the serial number it is entered in the register, and all the 8 small tubes are also marked with the same number. Tubes without number are not read. Numbering each tube is a lot of work, but it gives peace of mind. Also if there is shortage of techs in aparticular shift or on holidays the rack can be stored and read later.

But as Dansket said earlier we are waitng for automation to take us to 21st century procedures....hope that day will dawn soon.