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April 2024 Challenge

Yanxia

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Everything posted by Yanxia

  1. Yanxia
    I agree with L106 about the autocontrol. And what about the reverse group result? Rituxan is a kind of antibody preparation, maybe it can interfere the ABO group testing .
  2. Yanxia
    The DAT positive patient, some include antibodies have real specificity and others are mimicking antibodies. And the mimicking antibodies can be adsorbed by the antigen positive and negative cells( all or partial). It can be divided into two kind of antibodies, one is the antigen present on the patient's cells ,the other is the antigen is not present on the patient's cells. I think this patient maybe have the later mimicking antibodies. To prove this conclusion we can use some K negative red cells to adsorb the patient's serum or the patient's eluate, if we can remove all or part of the reactivity, then the guess is right. Sorry for my poor English, if it bring you some trouble to understand .
  3. Yanxia
    When we test a coagulate blood sample , we may get the positive C3d DAT result, this is not because the antigen and antibody reactive ,but because the blood coagulation is crosslinked with complement activation. In this question, I think all the positive result with screening cells and panel cells in RT with the same degree and neg in 37 degree C, we can get the conclusion that the antibodies is to a kind of high frequent antigen ,but not the autoantigen ,and the C3d DAT pos result can't give us the conclusion to autogentigen, too. Though lots of situation the conclusion is right,but I still think we need the autocontrol , it is necessary.
  4. Yanxia
    I don't think this conclusion is correct, because some disease can get the C3 DAT pos result ,such as DIC .
  5. Yanxia
    I think the heterozygous E and heterozygous c(R1R2) will be ideal. Because the fetus will not express homozygous E and c commonly,except some gene crossover or other gene change.
  6. Yanxia
    I am very interested in what is HTLA. Is it human T-lymphocyte antigens? And if it is why it will present on red cells? Thanks for any help.
  7. Yanxia
    Why not use auto serum add cells suspension be the negtive control instead of 6% Alb?
  8. Yanxia
    I think poly AHG can tell me the presence of complement dependent antibodies.
  9. Yanxia
    I had not encounter the IVIG cause HDFN, maybe it exist. I think to do the elution of the mother's DAT pos cells is a way to find the hemolysis reason.
  10. Yanxia
    I think the key is what is the relevant of DAT with the HDFN in ABO . We don't routinely do cord blood DAT if the mother is antibodies neg. And I don't think we need do DAT if the mom is neg and has no antibodies.
  11. Yanxia
    Sometimes pregnant can decrease the A and B expression of the RBCs. I think the reagent sensitivity is one reason and the pregnant is another.
  12. Yanxia
    I do the autocontrol along with crossmatch. The physician don't think this patient have auto-hemolysis, so he refuse to use incretion to lighten the maybe hemolysis during transfusion. He is afraid of minus effect on the illness, I understand him, and I have not the power to order him how to treat the patient.
  13. Yanxia
    Marilynm,we have not do the anti-IgG DAT,but the autocontrol in polybrene is pos, and autocontrol is neg in IS, so I think it is because the IgG autoantibodies. I don't think it is anti-H or anti-I, it looks like a kind of warm auto, but I don't know the specificity of it. My director want me to select a least incompatible cells to transfuse, I dare not to do this, if it is auto anti-D, the result maybe dangerous. What do you think?
  14. Yanxia
    We have encountered a patient just like this one. He is 56 years old, have not been transfused, liver disease. ABO type is B . Antibodies screening is positive with all three screening cells, aoto-control is pos ,too and is stronger than screening cells. It is on my night duty, we have no time to identify the antibodies , the patient is dead. His IS screening is neg ,polybrene and AHG is pos. I have a question, if he have not dead and need transfusion urgently, what will I do in the short time. To screen a least incompatible cell is safe or not? He has no the symptome of auto-hemolysis, I am not sure the antibodies is auto or allo.
  15. Yanxia
    I guess it is like B(A) phenotype, but the reaction is between the antibody in the reagent (of course not anti-A) and the antigen on the cells. Maybe you can test the patient's parents with this reagent. Because lots of antigen is inherited, and this kind of antigen is not popular in people.
  16. Yanxia
    I have encountered some pneumonia infants have cold antibodies.
  17. Yanxia
    I agree with SMW.
  18. Yanxia
    I am not sure what kind of test we should do to identify the antibody. I just want to say my idea. First ,we should do absorb and elute test with anti-D,anti-E,anti-C,anti-c, anti-e to identify the cell have not those antigens. The next we should use Rhnull and cell to test the serum of the patient to identify it just don't react with this kind of Rh cells.
  19. Yanxia
    Ckcheng mentioned a good method to make anti-A1. Do adsorb we need pay attension not excessive, because this can adsorb anti-A1, too.
  20. Yanxia

    Any ideas

    Yanxia replied to lef5501's topic in Case Studies

    What about the hematology disease, like hemoglobin disease or the erythrocyte membrane disorder?
  21. Yanxia
    When DAT is neg and autocontrol is pos, I think we can get this kind of result in two case, one is autoantibodies on cells is less and the other is false result. In enzyme technique the screening result is neg and in AHG it is pos, I think it maybe because the complements or the antigen is destroyed by enzyme.
  22. Yanxia

    Any ideas

    Yanxia replied to lef5501's topic in Case Studies

    I am very interesting what kind of method you use to do screening and crossmatches.
  23. Yanxia
    I am sorry for my misunderstanding in the first post. I agree with David Saikin. And I think maybe Ckcheng can use microscope to distinguish the real agglutination and mass caused by protein. If I am right this can tell us the DAT IgM result. As for the IgG you can use 2-Me to treat the cells to destroy IgM antibodies.
  24. Yanxia
    I will do elution test, and at the same time have another cells with the appropriate antigen we want to test to be a control.
  25. Yanxia
    I remember have read a book says:no evidence has been found that the least incompatible blood will live longer than the random one. In China we give this kind of patient the least incompatible blood, too. But I still want some evidence.
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