kell23

I was wondering how autologous units of patient's with an antibody should be handled. Is an AHG crossmatch needed? Does the unit need to be antigen tested if the patient has been antigen tested on their type and screen specimen? or can a 'historical typing' be used like the reference labs use. Any help is appreciated. Thanks.

LaraT23----you have always been helpful to me so please keep posting. I value your opinion and hope you will still be talking to all of us on BBK talk.

I included something to this nature in the procedure I just wrote. A friend had called CAP and they stated you had to QC expired panels but not the primary panel used since that was used in conjuction with the antibody screen. We would never used just expired cell panels and any questionable or inconclusive get sent to our reference lab. The Ortho screening cells hardly ever have positive cells for Lua and Jsa, that's were the problem comes in for our lab. Thanks for everyone's help.

What if you do not keep Lua and Jsa antisera on hand to QC expired panels?? Is AHG XM compatible acceptable?

Would anyone be willing to share their procedure for the usage of "Expired panel cells" for antibody identification? I was recently inspected by the State and have to put a policy in place to use expired panel cells. I know using expired reagents is not the best solution, but sometimes necessary depending on the number of antibodies a patient has and also for Lua's, Jsa's etc... We always perform an AHG XM, we are not AABB registered facility. Any help would be appreciated. Thanks.

We have hospitalists at our hospital and one wanted blood stat on a new patient who had an unknown antibody. She wanted to give uncrossmatched blood and I told her she should let us identify the antibody, she stated the patient would die if she didn't get the blood right now. I told her she might die sooner if we give her the uncrossmatched ....so her answer to that was.... 'just give her O Neg,,,..they do that on t.v. all the time"........so my reply was "they don't have antibodies on t.v.".........

Does anyone have a procedure they would like to share for comparrisons between gel and tube testing?

Thanks everyone who responded. I hope the techs I work with saw these posts. We did not give out type specific to our emergency patient, however one tech thought it was 'ok' to take the historical blood type. Good thing the other techs didn't take this techs advice. Thanks again everyone.

Hi, Just wondering what opinions others have about emergency releasing blood to a previous known historical ABO/RH type without having a specimen to verify the blood type. With the 'bad' practices of our ER with labeling specimens, I feel O NEG should be given until the patient is verified and an ABO/RH performed. How do others handle this situation? Thanks.

HTLA is "High Titer, Low Avidity" antibody

Hello, I'm new to BloodBankTalk. We are in the process of switching to gel testing. We recently had a patient that was a previous known Anti-FYA. We did our current tube method and the FYA was still detectable. My tech did a XM with FYA neg unit and thought it was microscopically positive with NHANCE. So I told her to try it in gel 'for fun'. It came out 1+ positive. We then did a gel panel and it showed the FYA but also had other reactivity that did not match any combo of antibodies. I asked the Manager for help and she said to titer out one of the cells that were positive not matching anything. So we did and got a titer of 2. My manager said that using gel, 2-1+ results would be enough to call this antibody a HTLA. I have not seen any literature on this so I'd like to know other techs experiences with gel titers for HTLA. Any comments and experiences would be appreciated. Thanks