When was the platelet transfusion and was the donor type O? Do I understand correctly that the eluate did not react with any O reagent cells, but did react with A1 cells? if your sample was drawn soon after the transfusion of an O pheresis platelet, you may be finding passive anti-A in the eluate. It would be pretty much all stuck to the patient's A cells so it is not interfering with the back type. Was the crossmatch by an antiglobulin method or immediate spin? An antiglobulin method might be able to pick up a small amount of anti-A in the patient's plasma, but one would expect an IS xm to behave like the back type. Did you test the patient with anti-A1 lectin? ...Or is this what you meant by Anti-A1 positive? Then the question becomes, "Did you test the eluate against A cells?"