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srichar3

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srichar3 last won the day on January 15 2019

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    Blood Bank Manager

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  1. We have received the following response from our AABB self assessment; 1. Std 1.3: It is not clear from documents submitted how your facility will transition to the new editions of the AABB Standards for Blood Banks and Transfusion Services. Please submit an SOP. We were requested to submit an SOP that covers transition to new versions of standards, so I sent our document control SOP that covers regular review of accreditation standards and updating policies and procedures when updates occur. I may be taking a too simplistic view of this but I don't really get what they are wanting. Or is this more to do with change control? Can anyone advise what they have in their SOP that covers this standard? Thanks
  2. This is what I thought also, as the cells had been re-spun maybe it changed the proportion of adult to fetal cells.
  3. The first and second cassette are the same specimen, the only difference was the washing of the cells between the first and second runs. I don't have an explanation as to why washing the cells changed the reactions from 0.5+ to mixed field but it seems to have increased the strength of the group B cells. There should be no incubation of cells and sera, it was performed on an analyser and they are immediate spin, even if slight delay in centrifuging the sera and cells should be separated until centrifugation. The APT test is alkaline denaturation, identifies cord blood from adult blood. The first specimen definitely had adult blood in it.
  4. Sorry to resurrect an old thread but as my question has already been discussed in this thread I thought it worth continuing here. I want to know if anyone has a technical explanation as to how a large amount of maternal contamination of a cord sample can occur? In the UK in my experience at all labs I've worked its always been practice to perform APT (NAOH) test on all cords that give the same blood group as the mother, I've always seen this as one of those tests you just do but seems a bit of a waste of time as its never positive. Last night we had a cord sample giving group O Pos result but with weak reactions in the B and AB wells. I advised the staff member to wash the cells and repeat, this then showed a strong mixed field, O and B. We requested repeat venous blood which was B Positive the mothers blood group is O Pos. APT test showed the first sample was not, or at least not all Neonatal blood. I've done a bit of a google search and although there are a lot of papers discussing contamination there isn't much that describes exactly how it occurs. I'm assured the samples are taken by double clamping then taking the blood with a syringe and needle. Thanks
  5. Would anyone be willing to share their policy that covers the above standard? Thanks Steve
  6. There one and the same thing, the difference been in the UK the lab staff are not involved in the collection process like seems to be the requirement under CAP. For example we have remote fridges, when the lab staff put the units in the remote fridge ready for the nurses to collect this is issued. Does that not fit your definition above of distribute? But at this point it is still not collected by the Nurses and may stay in this fridge for upto 48 hours before return to the lab if not collected. When we "issue"blood in the UK it is made available for the nurses, they then complete the final checks and sign the blood out alone not together with lab staff.
  7. I got into a bit of a debate today in a meeting with our IT regarding our blood bank module and wanted to see what others opinions are on what blood "issue" means. They were of the opinion that issue means the time the product is handed over to the nursing staff (collected), I disagreed as in my experience from working in the UK we consider issued the point at which the blood is assigned to the patient, crossmatched, labelled up etc and put in the fridge ready for collection. Collection is therefore as separate process not the same as issued. I do notice however that in the CAP standards there is no mention of recording time of collection from the lab by the nursing staff, only the time of issue so wondered if in other parts of the world issue is considered the collection process?
  8. I am just about to submit our self assessment for AABB, and wanted to know if anyone has any recent experience to share about the process for first time accreditation with AABB. When the inspection comes what should we expect? Regarding number of assessors, over how many days, what areas of the lab and the hospital will they want to inspect and see records from. Are the example questions in the self assessment a good representation of what they expect to see? Thanks
  9. I'm currently in the process of reviewing our ABO grouping discrepancies SOP and currently we do not have anything in there for investigation of AB subgroups, does anyone have an SOP they would be willing to share that covers these investigations? or can recommend a good guideline or reference for this information? Thanks
  10. Just checked the info and seems it does detect DVI, a bit sneaky of the rep to include this as extra when its not needed with the weak-D sera. "The blend of antibodies in this blood grouping reagent will directly agglutinate D positive red cells and may directly agglutinate red blood cells from most weak D and partial RhD including DVI. In addition the reagent will detect most partial D, and most weak D by IAT and, therefore, is suitable for RhD grouping of donor samples."
  11. We have extra Sera for DVI as Ortho dont do a DVI+ cassette, so at the minute were are doing both Weak-D and DVI on the RH-D neg cords. I'm pretty sure the Weak-D also doesn't detect DVI.
  12. Asking out of curiosity, is the Vision in the US using glass bead cassettes or are they still marketing gel in the US?
  13. When I asked Ortho in the UK for a Validation plan back in 2015 when I validated the Visions they wouldn't provide one as they said it wasn't allowed under ISO, UKAS who undertake the ISO accreditation in the UK confirmed this and said "verification" as they call it is the customers responsibility and the supplier should be impartial. Interestingly out here in the UAE the vendors run most of the validation/verification tests then analyse the data for you and give you the report which ISO inspectors here seem perfectly happy with. So much for international standards!
  14. Back in the days when we used to do blood groups in micro-titre plates manually, we always did the Du test as we called it then to pick up the weak D's. Since moving to gel technology in the beginning we picked up loads of D pos's that had previously been classed as neg due to the higher sensitivity of the Card methods. Since than I have worked at a few different labs and some have dropped the weak D tests and other have maintained them on newborns. I find the test a bit of a pain as the only time I find it positive now is when there is a positive DAT, I don't ever remember 1 occasion where the card D test was negative but the IAT D test was genuinely positive. Has anyone else who still does this test actually found a positive when the card Rh-d was negative?
  15. I thought the bottom barcode is probably product code, I was thinking maybe they didn't use ISBT compliant number due to copy write? But as you say no DIN would be a major issue both for compliance with ISBT standard and for checking procedures.
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