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srichar3 last won the day on January 15 2019

srichar3 had the most liked content!

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    Blood Bank Manager

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  1. And also my reason for asking which antigens are most likely, so I know which ones to try first rather than ordering them all.
  2. I'm looking into sourcing these from a company in Germany called inno-train who provide molecular blood typing products also, I believe from the name in the IFU's provided these are the same (imusyn GmbH & Co. KG.) that you mention above. My next question was going to be if anyone has any feedback on these techniques? imusyn rBGA Kits_v19_EN_RUO.pdf
  3. Column agglutination with glass beads instead of Gel from Ortho.
  4. We run auto control with all our panels and in these cases they are negative, were a women's speciality hospital in the middle east. I'm literally getting 3 or 4 of these a week and we have no reference service so mostly they are going unidentified. I'm trying to develop our identification protocols and source additional reagents to help ID these cases but struggling to find HFA negative cells out here. Just to add in most cases these are pregnant women.
  5. We receive a lot of antibody screens that tend to be positive in all screening cells, both with or without enzyme reactivity. In such cases what are the most likely culprits?
  6. Is there any Alinity HQ users who have results evaluation report for CAP survey FH9-B who would be willing to share a copy of the report? we are evaluating Alinity HQ and want to Alinity group means. Thanks
  7. Yes from the UK originally but now working in the UAE where we follow AABB and CAP standards. Are you from the UK also?
  8. In the UK its common practice, every hospital I have worked at follow this practice, it is strictly for life and death emergencies where there simply isn't time to lease with the blood bank. I haven't seen anything in the CAP or AABB standards regarding it that's why I was asking, I guess under US regulations/practices this isn't a followed practice then. Regarding the vending machines these are more a sort of remote electronic crossmatch than emergency O neg I believe.
  9. O Neg that is kept in a fridge that nurses/doctors can take in urgent situation without been crossmatched or issued to a patient.
  10. Just want to see how many labs out there use flying squad blood and if so how do you manage it? does your LIS have a process built in for managing such situations or do you have a manual process where the documentation is resolved later? Thanks
  11. Maybe I'm reading your question wrong but why do you need to use XM profile? we do antigen typing for all antigens on our vision and the Vision has profiles setup for all the sera it offers. Ortho also has the option for other manufacturers sera and these can be setup in the UDP (User defined protocols). You have to titre the sera to ensure it has a tire of less that 1:1024 as this is what Ortho claim is the limit of their analyser for carry over.
  12. Found an old Bio-Rad card that is still in date so decided to give it a try in that and its shows a plane old B Pos! But the Ortho gives a consistent 1 or 2 + A reaction in 3 different cassette types. Awaiting an explanation from Ortho.
  13. The A2 was done in Gel, or rather glass beads as Ortho is out here. The left well is A2 and the right is A1 so clearly weaker reaction with A2 but still reacted. I also repeated the tube method and when I did it, A1 gave strong reaction and the A2 was barely visible by eye by but large agglutinates observed microscopically. Any suggestions for further tests I can do on this one? or would genetic testing be the only way to resolve this now?
  14. I would have to check with the tech who performed it.
  15. In your experience does the fact the Anti-A is reacting with A2 cells rule out A subgroup of A been present?
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