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srichar3 last won the day on January 15

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    Blood Bank Manager

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  1. When we did plasma exchange and had a single SAHARA we used to have to start thawing overnight to get it ready in advance, they claim 6 bag capacity but in reality I found it was hard to fit 6 in. One site I worked at that was a major trauma center and did heart and lung transplants and ECMO had 2 for this reason.
  2. I have used the Sarstedt SAHARA III for many years and they are very good, with regards to limitations I am not aware of any as such, they use dry heat to thaw the plasma and I don't ever remember one breaking down in the 3 different labs I have used them in. Maintenance is limited to wiping down the unit every week and cleaning out when you get a burst bag but they have a tray in the bottom to catch any leakage, I would take one any day over any water bath options. My current lab has an Helmer, I find it very slow compared to the SAHARA.
  3. Are BPL still going? I gave up on them in the end, so many supply issues over the years, the only real advantage of BPL over the other suppliers was the fact they did a 250iu then they stopped producing that to concentrate on the 500 and 1500's. Plus the fact it was a lot cheaper than the CSL product.
  4. Worth noting not all preparations can be given IV, it depends on the filtration methods used. One of the main suppliers in the UK (BPL) could only be used IM, Rhophylac by CSL Behring can be administered IV.
  5. http://grifols.com/documents/10192/4198468/brochure-mdmulticard-en/fc571fca-f5e0-4b8c-97de-502b9a75f947
  6. Hi Malcolm I was aiming my comment at blood centers in other countries where PRBC's may be the only option, I don't believe we can order whole blood from our center here in the UAE and reconstituting PRBC's is the only option we have. I wish every blood service was a capable as NHSBT.
  7. I remember when I was in the UK a rep showing me a device they have for such a purpose, would have either been from Immucore or Ortho but cannot say for definite which one. If I remember correctly he said they were aimed at countries that require bedside checking of ABO prior to transfusion so there must be some countries out there where this is a requirement and there is a market for it. The device he showed me looked very much like the Diamed malaria strip test, a Elisa in a clear plastic case that gave bands for the positive reactions.
  8. When investigating grouping errors when antibodies with wide thermal range are present such as Anti-M reacting at RT and 37. What lengths do you go to to confirm the reverse group, for example if the screening cells are incorrectly positive I,e group B forward group reacting in the B cells and confirmed Anti-M reacting at room temp, do you just assume the Anti-M is responsible for the false positive reaction or do you go out of your way to find M negative group B-cells to confirm negative reaction in the back group. I go with the latter but all my staff seem to think I'm mad asking them to confirm this, just interested to see others approaches. Thanks
  9. If you PM me your e-mail address I would be happy to share our SOP with you. We don't wash the red cells though we only remove the supernatant then reconstitute with FFP.
  10. Hi Malcolm, according to the red book whole blood is used in the UK for exchange TX with removal of some plasma to increase the HCT. Is this not the reason why reconstituted PRBC are not been used as the end product is the same? But for labs that don't have access to whole blood reconstitution would be required to remove the additives and correct the HCT.
  11. When performing double exchange is the additive solution and anticoagulants not an issue? Plus removal of any potential residual AB antibodies if using group O unit with non group O patient? This was always my understanding of the justification for reconstituting.
  12. No you can buy inline filters for post storage leukocyte reduction, our blood supplier does not offer leukocyte reduction of platelets unless they bare apheresis units but they are not always available so we sometimes have to resort to using these filters. https://www.terumobct.com/imugard "MUGARD III-PL for Platelets The IMUGARD III-PL filter is a hard-housing filter designed to remove leukocytes and microaggregates from platelet preparations. Each filter system is equipped with a spike, clamps and tubing. The filter housing material is semitransparent to make monitoring the filtration process easier. Available in lab and bedside versions, the IMUGARD III-PL features a bypass line on the lab version to remove air from the transfer bag. The bedside version features a drip chamber and a roller clamp below the filter to adjust flow to the patient. Filters platelet concentrate for volumes equivalent to platelets produced from six Buffy Coats Offers greater than 90 percent platelet recovery" The efficiency is reported to be not as good as pre storage with these but this is not an option we always have. My issue is currently we are giving the filters to the nurses to do at the bedside which I don't feel is the best option and I would like to bring it into the lab, mainly because of training as its much harder to train all the nurses to do it properly than it is for the lab and of cause the lab can QC the process which would be impossible at the time of administration.
  13. Are there any sites that receive platelets non Leukocyte reduced and then perform the leukocyte reduction on site? If so do you do this in the laboratory prior to issue to the wards, or do you provide the nurses with the filter for them to perform at the bedside? Thanks
  14. But what temp do you consider acceptable for return? what evidence did you use to validate this? If you state it must still be at 1oC to 6oC, you may be throwing units away that are still fit for use. As CAP are asking for a validation then it needs to be evidence based, opting for 1oC to 6oC would be the easy option but I dont want to be throwing away units that we can still use. Are there any standards from AABB regarding this? I have a copy in the post but not sure it will come before our CAP inspection next month. Thanks Steve
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