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srichar3 last won the day on January 15

srichar3 had the most liked content!

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    Blood Bank Manager

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  1. I'm currently in the process of reviewing our ABO grouping discrepancies SOP and currently we do not have anything in there for investigation of AB subgroups, does anyone have an SOP they would be willing to share that covers these investigations? or can recommend a good guideline or reference for this information? Thanks
  2. Just checked the info and seems it does detect DVI, a bit sneaky of the rep to include this as extra when its not needed with the weak-D sera. "The blend of antibodies in this blood grouping reagent will directly agglutinate D positive red cells and may directly agglutinate red blood cells from most weak D and partial RhD including DVI. In addition the reagent will detect most partial D, and most weak D by IAT and, therefore, is suitable for RhD grouping of donor samples."
  3. We have extra Sera for DVI as Ortho dont do a DVI+ cassette, so at the minute were are doing both Weak-D and DVI on the RH-D neg cords. I'm pretty sure the Weak-D also doesn't detect DVI.
  4. Asking out of curiosity, is the Vision in the US using glass bead cassettes or are they still marketing gel in the US?
  5. When I asked Ortho in the UK for a Validation plan back in 2015 when I validated the Visions they wouldn't provide one as they said it wasn't allowed under ISO, UKAS who undertake the ISO accreditation in the UK confirmed this and said "verification" as they call it is the customers responsibility and the supplier should be impartial. Interestingly out here in the UAE the vendors run most of the validation/verification tests then analyse the data for you and give you the report which ISO inspectors here seem perfectly happy with. So much for international standards!
  6. Back in the days when we used to do blood groups in micro-titre plates manually, we always did the Du test as we called it then to pick up the weak D's. Since moving to gel technology in the beginning we picked up loads of D pos's that had previously been classed as neg due to the higher sensitivity of the Card methods. Since than I have worked at a few different labs and some have dropped the weak D tests and other have maintained them on newborns. I find the test a bit of a pain as the only time I find it positive now is when there is a positive DAT, I don't ever remember 1 occasion where the card D test was negative but the IAT D test was genuinely positive. Has anyone else who still does this test actually found a positive when the card Rh-d was negative?
  7. I thought the bottom barcode is probably product code, I was thinking maybe they didn't use ISBT compliant number due to copy write? But as you say no DIN would be a major issue both for compliance with ISBT standard and for checking procedures.
  8. Thanks for reply How do you find the pHix? What Ph does it give? and do you ever have to adjust it? ?
  9. Currently we are using 4 x 4 labels for our aliquots as per ISBT standard. These are too big for our neonate bag and attaching them is messy. I have seen some examples of 4 x 2 labels been used that would be perfect size however I'm concerned about compliance with ISBT standard. Can anyone tell me if such labels are acceptable under ISBT128. I have checked the standards and it always referances the 4 x 4 label and there is not mention of 4 x 2. Also all examples of these labels I have seen are missing unit number. This seems like the most important number to me, how can it be dropped from the label? How can the checking process be completed without unit number been shown? Thanks
  10. Does anyone make there own buffered saline? we have just switched to using the Ortho Vision and our local Vendor recommended normal saline not buffered. I have previously used the Vision in the UK and we were always advised to use PBS Ph7.2 and always did, so I was surprised by this advice. Since starting to use the Vision we have noticed a significant number of Cord blood samples with strong Pos DAT and Ctrl well positive, and C3 when checked with the Mono DAT card. When I asked the vendor regarding this he told me this was normal and to be expected, however I told him I don't believe it is as in my 2 previous years of using it I had never had a single case of this. I requested they contact Ortho regarding the issue and they came back with checking saline Quality/Ph! The Ph of our normal saline is 4-7 not within specification, the issue I have is that the only PBS I can get hold of here is for molecular biology use and around $100 a liter, as we are using about 150L a month this is not feasible. The ingredients to make PBS are cheap and its easy, or alternately we can just make the buffer to add to the saline. My concern is about QC and if we will have any issues from CAP/ISO,AABB etc. Just wondered if anyone else is doing this and if they have had any issues? Alternately I see Immucor make a product call Phix, that can be added to saline as a buffer, although more expensive this would be far cheaper than the PBS. does anyone use this product and have any feedback? There is also a powdered PBS product from a company called RPI which is the right specification and cost effective however they state that its a research product only, has anyone ever use this? Thanks
  11. We were provided the attached document by Ortho regarding saline specification. PH saline.pdf
  12. I know this is a bit late but I have recently validated titer on the Vision and found it to have at most only a 1 titre dilution difference to Bio-Rad gel cards. Some higher some lower but most agreed. I was pleased with the outcome, just a little disappointing that the only result you get is "Done" and it doesn't give an interpretation hence manual interpretation and entry is required.
  13. JAT is the automated survey. I think J is a manual only survey. I wasn't really discussing simply asking if there are know issue with CAP samples on Ortho Technology, as certain technologies do have know issues with EQA specimens. As this analyser isn't in use yet we are reporting the results from the IH anyway.
  14. I carnt really see any other explanation for it and would be interested to hear from any other Ortho users who have this latest CAP. Yes the backgroup was clear on Diamed/Bio-rad and they too had a c Poitive A cell, I tried the same cells on Ortho and it was Poitive, then switched to a screened A cell that was c Neg and it was clear. I didnt panel the antibody but going off the screening cell result it can only be anti-c.
  15. It could be that the cells are coated but not sufficient to get a positive result. However when you perform the eluate on a large enough number of RBC'c the amount of IgG removed from these red cell's is then enough to coat and cause a positive reaction on the screening cells. Also the cells from the DAT will be heterozygous cells, hence you will get a stronger reaction from screening cells that are homozygous if sufficient antibody is eluted to coat them.
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