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srichar3

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srichar3 last won the day on January 15 2019

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    Blood Bank Manager

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  1. Maybe I'm reading your question wrong but why do you need to use XM profile? we do antigen typing for all antigens on our vision and the Vision has profiles setup for all the sera it offers. Ortho also has the option for other manufacturers sera and these can be setup in the UDP (User defined protocols). You have to titre the sera to ensure it has a tire of less that 1:1024 as this is what Ortho claim is the limit of their analyser for carry over.
  2. Found an old Bio-Rad card that is still in date so decided to give it a try in that and its shows a plane old B Pos! But the Ortho gives a consistent 1 or 2 + A reaction in 3 different cassette types. Awaiting an explanation from Ortho.
  3. The A2 was done in Gel, or rather glass beads as Ortho is out here. The left well is A2 and the right is A1 so clearly weaker reaction with A2 but still reacted. I also repeated the tube method and when I did it, A1 gave strong reaction and the A2 was barely visible by eye by but large agglutinates observed microscopically. Any suggestions for further tests I can do on this one? or would genetic testing be the only way to resolve this now?
  4. I would have to check with the tech who performed it.
  5. In your experience does the fact the Anti-A is reacting with A2 cells rule out A subgroup of A been present?
  6. I meant it in the sense that it's an antibody reacting with the A antigen rather than another cold reacting antibody reacting with another antigen on the Acells been the cause. However as per my original post it did react with A2 cells this along with the strength of the back group was why I was questioning and AsubB.
  7. I tested the back group against 4 of each ABO group, Reacted 3+ with all A and AB samples and Negative with group O and B so its defiantly an Anti-A and not another cold antibody interfering. Also did A1 lectin and that was neg..
  8. AABB mandates the giving set are Pyrogen free, it appears the ones we use are Endotoxin free however they have advised that this is not in compliance with the standard as not all Pyrogens are endotoxins. Just curious what giving set other site that are AABB accredited are using? Thanks
  9. But going off the AABB technical manual it seems the back A cell reaction is too strong for a subgroup, and if a subgroup other than A2 it should be 1+?
  10. We have a case at the moment, a patient with no history, blood group by Ortho Cassette method is Anti-A 2+, Anti-B 4+, Anti-D 4+, CTRL Neg, A1 Cells 3+, B Cells Neg. Tube group gives B Pos and the Anti-A is totally clear Microscopically not even any apparent rouleaux, however when repeated at 4°C some small agglutinates were visible. Washing the cells yielded no change in the Anti-A reaction and auto control is negative in poly cassette as is the Antibody Screen. Back group tested with A2 cells also Positive. Is there any further tests that should be considered to try to ascertain the correct blood group? If not how would you report the blood group? Is this likely an A Subgroup B? If so would genetics be the only way to confirm this? Thanks
  11. The BSH guidelines from the UK that I've just checked state every 12 hours, but they do acknowledge there is a wide variation in the recommendations for this internationally, so maybe there is other recommendations used in the US?
  12. Do you have any reference for that? I was under the impression a giving set could be used for up to 3 units of PRBC and only needed to be changed if there is a delay between one unit and the next. Cant find the reference for this at the minute but I'm pretty sure this is stated in either CAP or JCI. Just check AABB and they don't seem to have any standard on it.
  13. The AABB standards state5.1.6.5.1 A unique identification shall be affixed by the collecting or pooling facility to each unit of blood, blood component, and attached containers, or a tissue or lot. This identification shall not be obscured, altered, or removed by facilities that subsequently handle the unit. If I understand this correctly this means that the original DIN of all received components must not be over written. When reconstituting PRBC's for exchange transfusion, ISBT guidance allows you to either assign your own facility DIN or retain the DIN from the original PRBC unit. If you issued your own DIN does this standard mean you must still show the original DIN? Could this not cause confusion with medical staff during checking as to why 2 DIN's are displayed? If you retained the original DIN, the product code would need to change and the expiry date, is it acceptable to just cover over these portions of the original label with the new parts of the label? (product code and description and expiry date) Leaving the original DIN and blood group visible on the label?
  14. I have the same question, does anyone have any input on this? My first though is the blood group, but so far we have only ever done 1 and both mother and fetus had the same blood group. Would the alkaline denaturation be an acceptable method?
  15. I've been looking for some guidance on the disconnection and re-connection of blood transfusions, the only thing I have found so far is that if there is a delay between giving two components then the giving set must be changed. Which would imply the same if a bag was to be disconnected then reconnected and I would have thought removing the giving set from the now opened port would be a contamination risk? Is anyone aware of any other standards that this would fall under? Regards Steve
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