Hi👋
I am solving case study of patient with AIHA (consistent with lab findings). DAT is +ve in IgG only. Antibody screen and panel is pan reactive in AHG (& enzyme-treated RBC) . After auto-adsorption, defined reaction was appeared in AHG but still panreaction in enzyme-treated RBCs.
My question is, is the reaction of enzyme-treated RBCs with auto-adsorbed plasma still due to incompletely adsorbed auto-antibody? Is it IgM, since some reactions are absent in AHG? How can this be resolved, please?
I have searched the internet without success, and I found this forum during my search.
Thank you 👋🙏