Tabbie

Can you expand on the IgG 2 and/or IgG4 rational/theory ? Was the DAT negative for these patients and can you give an explanation to why ? Thanks

In regard to the low affinity antigens it seems that DAT are usually negative as described because these antigens do not fit well with their cognate antibodies and HDFN/HTR is rare. Can any distinction be made in regard to positive/negative DAT results for high prevalence antigens ? The 901 series seems to cause severe HDN/HTR (Anti-Vel) and MAM describes DAT + but no HDFN with IgG1 and IgG 3 ? Thanks

What if the emergency o negs are taken for a patient with an anti-c antibody ? What protocols do you have for patients with antibodies who maybe are from another hospital (not on your system) and want for example group specific uncrossmatched ? Do you use concession/declaration forms before they take the O negs/group specific or can they just take them ? Thanks

Good points I used the wrong description. Can you clarify the use of frequency and incidence are synonymous with prevelance. Assuming so and that the 700 series is different collection only because they do no belong to any known blood group system ? Thanks

Does anyone use Alsevers instead of Cellstab and why which test would it be used for ?

Now reading the JPAC 1.2: Specifications, performance evaluation and quality control of blood grouping reagents and lab reagent inserts 😀 there seems to be a mix of human and murine products just wondering for example why anti-Lea is made from murine IgA as it’s biochemistry is built on type 1 chains can a human equivalent not be made ?

Thanks for all that detail much appreciated I will read about auto anti-E Happy Easter 🐣

Thanks Malcolm Is there a specific reason why ? There was a post and correct me if I am wrong as I can’t seem to find it again about crossmatching that was negative in patient with anti-E given E antigen positive units. Reading Daniels Human Blood groups Anti-c antigen sites are more than anti-e together with reverse grouping temp and no enhancements ?

Having read the BSH guidelines 4.7 about grouping anomalies “ reagents may cause IgG antibodies such as anti-c to be detected” Has anyone seen this or any other antibodies anti-e and what reagents did you use (A1, B cells typed as cde) ? Thanks Guidelines-for-pre-transfusion-compatibility-proceduresin-blood-transfusion-laboratories.pdf

I just answered this question. My Score PASS

So would u rule out the C and E if the anti-D reactions were not conistant in strength ? And would this apply only to the IAT or papain (enzyme) non reactive panel cells or both ? Could dosage not occur in that instance? Thanks

Could you post the link to these guidelines? Thanks

Hi Malcolm Can you expand on 'no longer see in vitro haemolysis' in EDTA samples. So if the patient had a transfusion reaction with in vivo haemolysis are you saying in an EDTA sample you would not see this haemolysis? Thanks

https://www.b-s-h.org.uk/guidelines/guidelines/validation-and-qualification-including-change-control-for-hospital-transfusion-laboratories/ This document has templates at the back and risk assessment info too Allard_et_al-2012-Transfusion_Medicine_Validation_guidelines.pdf

Just reading a the Gamma ELU-KIT 11 Immucor insert just says it may result in the dissociation of low-affinity antibody during the wash procedure, leading to an eluate containing no activity or less antibody than expected? It does describe final wash should be tested in parallel with eluate. I will read the references and get back to you with more info Thanks

Does anybody have issues with eluate washing with saline which can remove low prevalence antibodies ? or do most people use the low ionic strength solution? Thanks

I heard about it at IBMS Congress Although BSH guidlines are good this looks at the human factors too in a process I have a PowerPoint too in relation to blood transfusion if you give me your email I will send it to you https://www.caa.co.uk/Safety-initiatives-and-resources/Working-with-industry/Bowtie/About-Bowtie/Introduction-to-bowtie/ Thanks

Great thanks for that feedback 😀

Can anyone explain what the CD treated cell test is ? Thanks

Sorry gagpinks I can’t share as we don’t get anything interesting am afraid. I will email you. Ta

To clarify it stated it may be of value to perform IAT not that it was a standard test and that history of Prophylactic Anti-D injection should be confirmed. Thanks

I thought not just needed clarification

Just reading about using IAT test at room temp to help distinguish Immune and Prophlactic anti-D. Does any one do this routinely together with quantitation ? Thanks

Hi All Just wondering if anyone has gone through validation of the IH500 analyser and if they could share any issues that may have occurred ? I am currently writing the Validation policy/plan and would be good if we could prempt some of the IQ before it gets to the PQ. I am following the BSH guidelines. Thanks

Hi All Just wondering if anyone has applied the bowtie method of risk assessment in the context of transfusion labs and what significant 7 elements they have chosen as the basis ? Thanks