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AMcCord, Who manufactures your reagents? We use Immucor and they state you "may" wash the cells, but it isn't required. We do wash cord bloods 3 times. The staff in family birth usually get as much blood on the outside of the tube as in the tube! Gross!

Well, there are two possibilities that are comparatively common. The first is that the patient has a positive DAT for no particular reason, as do a certain number of healthy donors, in whose circulation red cells exist normally, with no shortening of the normal life span. The other is that the dialysis machine has been cleaned/sterilised by the use of formaldehyde, where they can make anti-Nf, but I doubt this one, as the effect has been known about now for about 44 years, and the antibody is not normally an auto-antibody (so you would not expect a positive DAT) and it is usually not active above about 20oC, but it remains a possibility. I'm certain that others will have better answers to your query.