I don't know about others but gel is the not the end-all, be-all in antibody studies. For instance, if I am doing an warm auto - I have never been able to absorb all the warm ab out of the specimen if I test in gel. I always go with a PeG autoabsorption and do my studies in tubes rather than gel. I don't think I am going to miss any signficant allosensitization using tubes for this scenario. I still have referrals that use tubes and 22% albumin or LISS routinely, I like to be able to reproduce their results using the modalities that they use (when necessary). Sometmes gel is just way too sensitive and if I back off the sensitivity I can get a clear cut result. I feel the same way about the capture technology - sometimes it is way too sensitive. Tube testing, I believe, is still the standard of care regardless of what the vendors are saying or even that the majority of users use the newer technologies. I also do NOT run all specs in the cold - only if the studies indicate a likely cold reacting moiety.