PFA-100 QC frequency

[SMILLER]

For the PFA-100, we have been running wet QC (known normal) every week (or trying to remember to, anyway).  When we get a new shipment, we also run more extensive QC including abnormals at the time.

 

It seems as though we should be able to drop the once a week requirement. We are not even sure why we started it years ago when the analyzer was new.  Anyone have any comment on QC requirements for this analyzer?

 

Thanks, Scott

[nziegler]

we run "qc" once every 30 days (more accurately, whenever we get a normal patient - seeing as how we only test 3-5 patients per month).  we also qc a new lot number.  we'll draw a co-worker that we know works if need be.

[SMILLER]

No abnormal QC at all?  We use a aspirin spiked normal for the EPI, and EDTA plasma for the ADP.

 

Thanks, Scott

[nziegler]

We only report up to 160 seconds, so we don't do any abnormal controls.

[falgunijoshi]

we report Collegen / EPI and ADP in sec with comments as:

Platelet disfunction detected by the PFA-100 may be acquired, inherited, or

induced by platelet inhibiting agents. Uremia may cause platelet disfunction.

Hematocrit < 35% causes prolonged closure time, and HCT < 20% causes significantclosure time. Studies show that significant increases in closure time when the

count of functionally normal platelets is reduced from 200,000/ml to 50,000/ml

with a constant hematocrit.

The following are expected patterns observed with the PFA-100 tests on normal

subjects and subjects with various disorders.

Reference Interval (sec)

Collagen/Epinephrine 95-185

Collagen/ADP 65-120

Normal ASA vWD Glanzmann's Thrombasthenia

Col/EPI Normal Abnormal Abnormal Abnormal

COL/ADP Normal Normal Abnormal Abnormal

I hope this will help u

[jojo808]

We follow reporting very similar to "falgunijoshi's" facility. As for QC, we run it at least every 6 months or whenever we get a new lot or shipment. I believe the 6 month came from the manufacturers recommendations.