lacs Posted September 17, 2011 Share Posted September 17, 2011 Multi-racial patient in May 2011 APOS, R1rK-Fya-Jkb-S-, reacting with all cells, including auto, and reacting stronger w/D+ cells.Eluate pan-reactive w/all screen cells in tube.Patient’s DAT negative reactive w/eluate and plasma=WAAD typing w/BioClone 4+ IS; 3+ W/Immucor/GammaAdsorption w/Rh- cells left behind DPanel against Cord cells (to rule out LW)-negativeDTT treatment of red cells against adsorbed plasma–stayed positive-true D.Aug 2011, reactions match a typical WAA exactly. Removed w/Rh+, pheno-similarSept 14 workup-Looks like a warm. Adsorbed against Rh looks like anti-E and D, Lua left behind.Is this WAA w/D-variant? Anti-LW or WAA w/D, E and Lua? Why was D disappearing w/2nd workup? Patient is Rh+ Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 17, 2011 Share Posted September 17, 2011 Please could you tell me with what reagent(s) you performed the DAT on the original sample? Link to comment Share on other sites More sharing options...
lacs Posted September 17, 2011 Author Share Posted September 17, 2011 Multi-racial patient in May 2011 APOS, R1rK-Fya-Jkb-S-, reacting with all cells, including auto, and reacting stronger w/D+ cells.Eluate pan-reactive w/all screen cells in tube.Patient’s DAT negative reactive w/eluate and plasma=WAAD typing w/BioClone 4+ IS; 3+ W/Immucor/GammaAdsorption w/Rh- cells left behind DPanel against Cord cells (to rule out LW)-negativeDTT treatment of red cells against adsorbed plasma–stayed positive-true D.Aug 2011, reactions match a typical WAA exactly. Removed w/Rh+, pheno-similarSept 14 workup-Looks like a warm. Adsorbed against Rh looks like anti-E and D, Lua left behind.Is this WAA w/D-variant? Anti-LW or WAA w/D, E and Lua? Why was D disappearing w/2nd workup? Patient is Rh+Hi Malcolm,I don't have the exact info, but we use Immucor AHG, IgG and C3 for all WAA workups Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 17, 2011 Share Posted September 17, 2011 The reason I asked is because, on some occasions, the auto-antibody can be largely IgA.I'll have another think! Link to comment Share on other sites More sharing options...
lacs Posted September 17, 2011 Author Share Posted September 17, 2011 The reason I asked is because, on some occasions, the auto-antibody can be largely IgA.I'll have another think!Hi Malcolm,What about the D pattern after adsorption with D- cells then? It comes and goes. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 19, 2011 Share Posted September 19, 2011 Hi Malcolm,What about the D pattern after adsorption with D- cells then? It comes and goes.Yes, that's the bit that is giving me acute brain ache!I would think that the answer could be as simple as the auto-antibody is mimicking an anti-D, is then broadening in specificity, and then narrowing again, and so on, depending upon the patient's condition; but it may also not be that simple!!!!!!!!!!!!!!!!!!!:confuse::confuse: Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 Yes, that's the bit that is giving me acute brain ache!I would think that the answer could be as simple as the auto-antibody is mimicking an anti-D, is then broadening in specificity, and then narrowing again, and so on, depending upon the patient's condition; but it may also not be that simple!!!!!!!!!!!!!!!!!!!:confuse::confuse:Thank-you Malcolm, I am sticking to that theory also since we ruled out ITP, passive antibody and D variant. Link to comment Share on other sites More sharing options...
galvania Posted September 19, 2011 Share Posted September 19, 2011 Can I just see if I have understood correctly? Your patient is reacting (I presume in Coombs) to all panel cells and his own auto control, but cord cells are negative and his DAT is negative? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 19, 2011 Share Posted September 19, 2011 Can I just see if I have understood correctly? Your patient is reacting (I presume in Coombs) to all panel cells and his own auto control, but cord cells are negative and his DAT is negative?If you' re right Anna, and I haven't checked, sounds like an alloanti-I in an adult ii?????????????? Link to comment Share on other sites More sharing options...
Yanxia Posted September 19, 2011 Share Posted September 19, 2011 I am very interesting in this post, but because my English is not so good, I can't understand it well.I have difficult to understand what is the meaning of APOS, w/D+ , plasma=WAA,pheno-similar. Help is expected, thanks in advance! Link to comment Share on other sites More sharing options...
Rh-fan Posted September 19, 2011 Share Posted September 19, 2011 I am very interesting in this post, but because my English is not so good, I can't understand it well.I have difficult to understand what is the meaning of APOS, w/D+ , plasma=WAA,pheno-similar. Help is expected, thanks in advance!I agree (as also not englisch speaking by nature), the start of this topic was a little bit hard to read because of the short hand writing.According to sugestion af the 'allo' anti I, I would have aspect that the antibody was also reactive at lower temp (and I see only report of warm antibodies) and it dont explane the positive DAT (although that could also be something else).You have used to anti D reagents, is that how you ruled out an RhD variant?I agree with Malcolm that "mimicking" is the only explanation for the come and go af the antibody, that suggest an auto antibody (LW or D does not matter) Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 19, 2011 Share Posted September 19, 2011 I am very interesting in this post, but because my English is not so good, I can't understand it well.I have difficult to understand what is the meaning of APOS, w/D+ , plasma=WAA,pheno-similar. Help is expected, thanks in advance!shily, both you and Rh-fan are right - we do use an awful lot of "jargon", and sometimes even people who speak English do not understand one another - particularly when one is speaking UK English and the other US English!!!!!!!!!!!!APOS probably means group A, D Positive (for someone who, like me, also does not like this terminology, read Peter Issitt's books!), w/D+ probably means Weak D (Weak D+ means nothing, as there is no such thing as anti-Weak D), plasma = WAA probably means the plasma contains a warm auto-antibody, and pheno-similar means that blood was given that was not positive for an antigen within the Rh Blood Group System that the patient themself lacks - for example, an Rir patient would be given E- blood, but could be given R1R1 or rr blood, both of which are E-, but neither of which is Rh identical. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 19, 2011 Share Posted September 19, 2011 According to sugestion af the 'allo' anti I, I would have aspect that the antibody was also reactive at lower temp (and I see only report of warm antibodies) and it dont explane the positive DAT (although that could also be something else).But an alloanti-I can have a wide thermal amplitude, and not just be detected in the cold. I thought that, in the original sample, the DAT was negative? Link to comment Share on other sites More sharing options...
Yanxia Posted September 19, 2011 Share Posted September 19, 2011 Malcolm, you are a good teatcher, you explain it very clear, thank you very much.I think there is a strange result is the DAT, it is neg, but the auto is pos and the eluate is pos,too.I don't know the pos result is how strong, if it is weak, maybe because the antibody on the auto cells is too less to react in the DAT, and auto will add 2 drop of plasma and incubation to strengthen the react, and eluate will let the antibody in a less volume liquid to strengthen it too.Sorry, I am not agree with the result of allo anti-I, because the pos auto and eluate. Link to comment Share on other sites More sharing options...
Yanxia Posted September 19, 2011 Share Posted September 19, 2011 If the eluate is equal strength to all panel, I prefer the anti-D is alloantibody, because the plasma reactive stronger with D pos cells, if it is autoantibody show anti-D specificity, the eluate will show the same reaction.I think it is autoantibody add allo anti-D.Just a guess, if it is not right, please point it out, thanks. Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 The reason I asked is because, on some occasions, the auto-antibody can be largely IgA.I'll have another think!Hi Malcolm,We wouldn't be able to elute if off if WAA is IgA, would we? Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 Can I just see if I have understood correctly? Your patient is reacting (I presume in Coombs) to all panel cells and his own auto control, but cord cells are negative and his DAT is negative?Hi Anna, Sorry for my abbreviations. It's a long case and I wanted to shorten it:)The patient's DAT is positive. What I meant above was that patient’s EGA-treated DAT negative reacted with his/her eluate and plasma. This proves that what we see in the plasma and eluate is WAA. What we don't understand is that anti-D is left behind when adsorbing with Rh-phenotype similar red cells. In our experience, WAA with a specificity would be removed by either antigen positive or negative against that specificity. Also, this D pattern comes and goes. We ruled out LW even though cord result points toward it. Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 Would we be able to eluate anti-I in the eluate? How do we explain the D pattern then? Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 If the eluate is equal strength to all panel, I prefer the anti-D is alloantibody, because the plasma reactive stronger with D pos cells, if it is autoantibody show anti-D specificity, the eluate will show the same reaction.I think it is autoantibody add allo anti-D.Just a guess, if it is not right, please point it out, thanks.Hi Shily, The patient is Rh+. We ruled out D-variant, LW and passive anti-D. Where would allo-anti-D come from then? The patient does not have ITP with WinRho administration. Link to comment Share on other sites More sharing options...
lacs Posted September 19, 2011 Author Share Posted September 19, 2011 Thanks Malcolm for "interpreting" for my short-handwriting. I wanted to shorten it but at the end made it more confusing.Our Medical Director is looking into the patient's medical conditions and history. Perhaps that will shed some light on this case. Link to comment Share on other sites More sharing options...
Yanxia Posted September 19, 2011 Share Posted September 19, 2011 Thanks,Lacs. EGA-treated will destroy the Bg antigen, is it Link to comment Share on other sites More sharing options...
lacs Posted September 20, 2011 Author Share Posted September 20, 2011 Thanks,Lacs. EGA-treated will destroy the Bg antigen, is itHi Shily,It is correct but Bg antigens are leukocyte antigens of low incidence. Link to comment Share on other sites More sharing options...
christyn Posted September 20, 2011 Share Posted September 20, 2011 Iacs, Do you have a drug history for this patient? Even if he has not received IVIg there are some drugs that can cause anti-D like reactivity. Did you try doing a super-DAT? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 20, 2011 Share Posted September 20, 2011 Hi Malcolm,We wouldn't be able to elute if off if WAA is IgA, would we?Well, yes you would, but the important word in my post was "largely", because there is usually some element of IgG there as well, and, although you may not be easily able to detect this by DAT, as shily says, the elution technique has the ability to "concentrate" any antibody on the red cell surface, and so you may well be able to detect this in the eluate (thus making the entire case even more confusing!!!!!!!!!). Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted September 20, 2011 Share Posted September 20, 2011 (edited) Would we be able to eluate anti-I in the eluate? How do we explain the D pattern then?This is quite a complicated concept, but I'll have a go at explaining it (and probably completely mess it up - but here goes anyway).Early in the production of human IgM anti-D, if you catch it just at the wrong point in time, the anti-D is so poor at being specific it can mimic an anti-I. This is because there is a short part of the D molecule that shares moiety with the I antigen (I can't immediately put my hand on the paper about this - it is buried in the rest of the detritis on the desk in my office, but I will try to find it. It won't be straightaway though, because I'm lecturing all this morning) and, as you know, IgM antibodies made straight after primary response have a "wider specificity" than do later IgM molecules and IgG molecules.This was also true of many monoclonal IgM anti-D reagents in the early days (and may still be so, for all I know), and this was why it was always recommended that these reagents be brought up to room temperature before use - so that no false positive reactions were detected with I antigen in the cold when the patient was actually D negative.Now then, if your patient has continued to make this particular specificity (rare, but not unknown), it is entirely possible that this single antibody will react with all I positive red cells, but that this single antibody will have a greater affinity for D+ red cells. This would also explain why the "anti-D-ness" of the antibody comes and goes, with the condition of the patient.This explanation is in no way a "definitive answer" to this complex case, but it cewrtainly is something to think about.Mind you, of course, this goes entirely against my earlier theory that it may be an alloanti-I in an adult ii!!!!!!!!!!!!!!!!!!!!!!!!!!!!:no::no: Edited September 20, 2011 by Malcolm Needs Afterthought. Link to comment Share on other sites More sharing options...
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