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Running into problems validating Anti-C3 in buffered Gel


gene20354
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Some time ago I asked for advice on validating Immucor's Anti-C3d with Ortho's bufferd gel card. I got the help that I needed but I have run into a problem. I have tested 4 samples that produced weakly positive to 1+ reactions with anti-C3d in the tube but all were negative when I tested them in gel. I have been converting Immucor’s complement control cells to 0.8% and using them as my positive control. The control always works but I can't figure out why my samples are coming up negative. Has anyone experienced this?

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