Jump to content

Running into problems validating Anti-C3 in buffered Gel


Recommended Posts

Some time ago I asked for advice on validating Immucor's Anti-C3d with Ortho's bufferd gel card. I got the help that I needed but I have run into a problem. I have tested 4 samples that produced weakly positive to 1+ reactions with anti-C3d in the tube but all were negative when I tested them in gel. I have been converting Immucor’s complement control cells to 0.8% and using them as my positive control. The control always works but I can't figure out why my samples are coming up negative. Has anyone experienced this?

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now

  • Advertisement

  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.