QC AHG, C#/ Part II

[David Saikin]

I asked her what answer I would get if I called today? She laughed . . .

[NedB]

The reason we add check cells is to make sure we have removed enough protein in the preceding washes to eliminate that interference with the AHG. So, checking the reaction of Poly AHG with either Coombs Control Cells or Immucor's Complement Control Cells should be acceptable, no? And you wouldn't need both.

[profbaud]

We perform QC on Polyspecific AHG when we get a DAT order on an adult and its good for 24 hrs from the time it is done. We use 3 cells: 3% Selectogen I [Negative control], Coombs control cells [Positive control for IgG component] and C3 check cells [Positive control for the C3d component]. We spin the tubes at immediate spin and after 5 minute incubation. The Coombs control cells are 3-4+ at IS, the C3d cells can be 0-1+ at IS and 1-2+ at 5 minutes, depending on the age of the check cells and the SI cells remain negative at IS and 5 minutes and then positive when Coombs check cells added.

Hope this helps.

[NedB]

Poly AHG is now comprised of Anti-IgG and Anti-C3b,-C3d. In the old days, we used Poly AHG because it had other anti-complement components. We especially used it for testing babies. Now the Poly AHG from both Ortho and Immucor are identical in manufacture. Does anyone know what the other manufacturers' product inserts say?