SOP for the titration of hemagglutinins

[Sherif Abd El Monem]

Hi everyone, a colleague needs an SOP for the titration of hemagglutinins for LTOWB. Could you please share your SOPs?

Thanks in advance for all your help.

[Cliff]

This is just my opinion.

First, it's usually not a titer but a single-point dilution.

What dilution?  It's truly arbitrary.  Some go 1:50, some 1:100, others 1:200.  It depends on what the facility medical director will accept.

Your colleague should collaborate with their medical director to determine what they feel is "safe".  This cut-off will not prevent reactions, only minimize the risk.

Then from there, it should be a simple procedure of a single-point dilution, with a cut of 1+, 2+... or less.

[Bet'naSBB]

Our supplier titers all our WB and it's marked with </=200. We only go "farther" and do an extra dilution for Oneg LTWB that we set aside for pediatric trauma use - it needs to be <50