KB TESTING

[ESIZENSKY]

Does Sysmex have the capability to perform fetal cells count for KB?

[SbbPerson]

On 4/23/2020 at 9:23 AM, ESIZENSKY said:

Does Sysmex have the capability to perform fetal cells count for KB?

I think this question is suited more for sysmex IT tech support. I don't know about these days with modern day techs, but in my experience, it's the techs that perform the cell counts using a miller disc for the KB method.  If your facility uses 300 uL dose RhIg, you can use this simple equation: (%fetal cells x 50) / 30 = number of vials of RhIg required.

 

Remember to use the "fudge factor". 

 

Example:  1.3% fetal cells calculated on Kleihauer Betke stain

1.3 X 50 = 65 ml of fetal blood

65 ml/30 = 2.2 vials of RhIG required

When the number to the right of the decimal point is less than 5, round down    and add one dose.  Example:  If the calculation comes to 2.2, give 3 vials.

When the number to the right of the decimal point is greater than 5, round up    and add one dose.  Example:  If the calculation gives 2.8, give 4 vials.

 

source: https://hsc.ghs.org/obgyn-clinical-practice-guidelines/inpatient-obstetrics/kleihauer-betke-assay-interpretation/