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Showing results for tags 'antibody id'.
Found 5 results
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Okay so I was always taught to use the rule of 3, 3 positive reactions and 3 negative reactions for peforming an antibody ID. I was also taught to always use homozygous positive and negative cells whenever possible. Sometimes of course it is not due to low incident/high incident antigens. I do know you need to use a homozygous cell when performing "rule outs". What is everyone else's practices and thoughts as I need to clarify our current antibody identification policy. Thanks in advance.
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We have 1 antibody panel and the only type of Antibody ID we perform is a modified Antibody ID (1 rule out) for maternal anti-D when we have documentation that the patient received RhIG during the pregnancy. We have an inspector asking what we do for proficiency for this tsting. We subscribe to API for our proficiency but they only offer a general ABID proficiency. Can anyone share how they handle proficiency for this selective type of Antibody ID testing?
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I have read an article from last year about a lab that routinely runs a gel enzyme panel when they get equivocal results (i.e. antibodies of undetermined significance, AUS's, non-specific reactions) on gel screens or panels. About 25% of the time they identified significant allo-antibodies that otherwise would have been missed as they would have been ruled-out on the regular panels. Does anyone else do this? https://academic.oup.com/labmed/article/48/1/24/2666003 Thanks, Scott -
I am switching from an Immucor antibody panel to a Quotient antibody panel and need to do a validation. Does anyone have any suggestions regarding this? How many samples (both pos and neg) I should use or know of any references I can look at?
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We have a patient with a history of Lua. According to our policy, if her screen is negative (there are no Lua pos cells on our 3-cell screen panel), we check for reactivity with three Lua positive cells taken from our ID panels. If anti-Lua is showing up, we would release AHG compatible blood, if needed, without having to check at all for Lua antigen. If the anti-Lua is not showing up at all, we would have to order confirmed Lua neg units from our blood supplier (ain't got none of that fancy anti-Lua reagent here) and crossmatch those. Question: assuming the screen is negative and the three Lua positive cells from other panels are all positive with the patient's plasma, do we in this case have to rule out all the other antigens as well? (we use a 3 x 3 r/i r/o rule here) Scott
