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  1. BloodbankZ posted a post in a topic in Transfusion Services
    Okay so I was always taught to use the rule of 3, 3 positive reactions and 3 negative reactions for peforming an antibody ID. I was also taught to always use homozygous positive and negative cells whenever possible. Sometimes of course it is not due to low incident/high incident antigens. I do know you need to use a homozygous cell when performing "rule outs". What is everyone else's practices and thoughts as I need to clarify our current antibody identification policy. Thanks in advance.
  2. There are a number of well-read (and well published) posters here. I am wondering if any of you know what the most common cause of equivocal reactions that come upon during antibody ID? You know, the often weak reactions that are left over after all of the rule-outs are accomplished? Some are apparently the result of HLA interactions, but are most just junk reactions with otherwise unnamed insignificant antigens? More importantly, how many are actually "developing" antibodies to significant antigens? We do see, over time, patients pop up with only a few equivocals, and after a few transfusions, develop a full response to a significant antigen. I was just wondering if there is any data on how commonly this occurs. Thanks, Scott
  3. Mosaics posted a post in a topic in Case Studies
    Does anybody know of websites (other than Immucor or American Red Cross's SUCCESS) that have interactive case studies for working up antibodies? The inner nerd in me is itching to antibody puzzles frequently.
  4. Does anyone have any procedures that they can share for making up student specimens, either spiking plasma samples with antibodies but even more so, making up specimens for doing eluates? If there are some on this site already, sorry for the repeat, but I can't find them.

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