kitty1392

I have always done joint CAP/AABB inspections and the CAP portion is almost negligible. I don't see a huge pro to dropping CAP if you are already AABB, since the two are very rarely in disagreement, and AABB tends to have stricter guidelines.  

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I have always done joint CAP/AABB inspections and the CAP portion is almost negligible. I don't see a huge pro to dropping CAP if you are already AABB, since the two are very rarely in disagreement, and AABB tends to have stricter guidelines.  

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We do not perform antibody screens on babies, only a forward type and a IgG DAT. We use the mom's results when considering transfusion.  What is your particular concern for using one screen versus the other? If a mom has always had negative screens, never demonstrated an antibody, great, that was easy.  If the mom was previously demonstrating an antibody, even if she is negative now, you would still need to honor that antibody if transfusing mom or baby.  To me, it's no different than any other patient. Their current antibody screen is important, of course, but you still need to honor any previous antibody IDs. 
 

Do you have a reference lab that could send you any antibody positive plasma aliquots?
 
As for eluates, you could always incubate some anti-D with D positive red cells. I usually just use cells from a patient sample with a warm auto for training on eluates.

Whenever I review panels I look for patients with at least a 1+ antibody and grab all the old lab specs from other depts I can lay my hands on. I pool the serum/plasma and aliquot in 1-2 ml samples and freeze. They seem to keep their reactivity well at -28o. If they're weak you can always do a little demo on differences in sensitivity between LISS, PEG, enzyme, gel etc.
I agree about the monoclonal reagents; if I use them I warn the students to just cross out and look for the specificities and that the human-source ones don't react like that.
Quotient offers free or cheap deals on expired antisera for student use.

I don’t want to overstep the bounds of information from vendors on BloodBankTalks, but I’d like to clarify the information about Quotient’s support of schools. We have a program that provides in-dated products at low cost for MT/MLT and SBB programs and if we have any products that we are not planning to sell, such as short dated products or trial products, we distribute those free of charge to schools on a first come, first served basis. We also have a Competency Training Kit that provides antibody samples all ready for use.
Hope the information can help someone. Jeanne (Technical Director, Quotient Biodiagnostics)

I use the recipes below for students/new techs. We use mostly gel for testing, and these work pretty well most of the time. The Rh, K, and M are very consistent and we get 2+ reactions or better. I don't know what kind of reactions you would get with tube testing with the Rh and K. I've had problems with the Fy, so I only use it in combination with other antibodies. Sometimes I mix it up based on what our expired panels look like. If I'm feeling particularly evil (or want to stump a student who thinks they can solve anything), I will choose several antibodies that are very difficult to identify with a particular panel. We use mostly monoclonal antisera, and I try to use expired, but since I'm only using a drop or two, I don't feel bad about using in-date reagents. I either match the antibodies to an expired panel cell to use as "patient" cells, or use some segments from a phenotyped unit if one is available. I've tried making positive DATs with reagents other than D, but haven't had much success.


Elution: Anti- D, mixed field DAT
2 O Pos Segments and 2 O Neg segments
Put O pos segments in 12x 75 tube.
Add 1 drop of Anti-D, 2-3 drops of albumin and a little bit of saline
Incubate at 37 for 30 minutes
Add O neg segments

[*]Gel ab screen- Anti- C

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-C, 2 drops albumin

[*]Gel ab screen- anti-Jka

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-Jka, 2 drops albumin

[*]Gel ab screen- anti-K and Anti- c

Fill 12x75 tube 1/3 full of saline
Add 1 drop Anti-K, 1 drop Anti-c, 2 drops albumin

[*]Tube Ab screen – Anti-M

Fill 12x75 tube 2/3 full of saline
Add 2 drops Anti-M, 2 drops albumin

[*]Gel Ab screen - Anti-Fya, Anti-E, Anti-K

Fill 12x75 tube 1/3 full of saline
Add 2 drops Anti-Fya, 1 drop Anti-E, 1 drop Anti-K, 3 drops albumin