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Rh-fan

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Everything posted by Rh-fan

  1. Rh-fan
    I see your point Malcolm, but I think that when a sample is sent to a reverence lab, the reverence lab has to give the advise. In that situation we diside we can not rule out and say select S negative. The presence of multiple antibodies (or HFA) can never be the reason to rule out on heterozygous cells. You have to search for other cells or use other methodes to rule out, for example absorption. Last year we had a pregnant women with anti P+Pk+P1, and the father is K+ and E+. We do not have cells that are P- and E+ or K+. So have ruled out these antibodies several times during pregnancy with absorption. And the good news is she is now pregnant again!! Peter
  2. Rh-fan
    In method 1 and 3 you see no reaction with anti A,B is that a typing error or do I mis something?
  3. Rh-fan
    The patient has made all 'normal' allo antibodies and now has made a pan-reactive antibodies that needs 4 absorptions. I hope that this is an auto antibody and not a allo against a HFA. Peter
  4. Rh-fan
    Thank you all for this information, I am writing an abstract/case report about a patient who had made an anti Jkb after 72 hours and 4 days later had made an anti S (beside the anti E and anti Fya that were known already). And I was interested in the time in other labs. Peter
  5. Rh-fan
    So that can be up to 96 hours or is that not a good interpretation. If you draw the sample at 4 in the morning (at day 0) you can transfuse till day 3 at 24:00. That is 92 hours. Is this correct?
  6. Rh-fan
    In the Netherlands we have to repeat a antibody screening/identification after 72 hours, to see if there is new antibody formation. How is that in other countries, dou you also repeat the ruling out after 72 hours or after 48 hours? Peter
  7. Rh-fan
    In the case of Dansket I would not select K neg blood, because there is hint thet there is an anti K present (you can blame your reaction on the anti D). In a case where only the K+k- neg is reactive (and 2 or more K+k+ are non rective), I would look further for the anti K or select K neg blood. A few weeks ago we had a case like that, screening neg and crosmatch positive (was KK). Peter
  8. Rh-fan
    When you have ruled out. ABO, Rh and K is enough for us. Peter
  9. Rh-fan
    Shilly is right, The term anti A1 is not completly correct. I should have said anti A. I have the tendency to call these antibodies anti A1 because the person is group A (subgroep Ax or lower). In that case you can see a antbodies that is strong reactive with A1, weak reactve with the cells of the patient.
  10. Rh-fan
    The last 2 weaks we have had 2 patients with the blodgroup AB, but with very weak expression of the A antigen. (pointing to A3 or Ax). In the serum they both had a strong anti A1, also reactive at 37oC, and at RT also reactive with A2 cells. These patients have the same last name ( I think family, same region). After further looking we found 3 other (probably family members (name and region) we have had the last 8 years and al have the same A3B/AxB bloodgroup and a strong anti A1. The B antigen is normal (not weakend, determind in titer). Is it coinsidence that they all have also a B antigen, or is this more like a B(A), cisAB or something else? Any sugestions? Peter
  11. Rh-fan
    What do you meen with "probeble HTLA", is that only weak reactions and a high titer, or do yo have more info. If most allo's are ruled out then we would only select for RhCcEe and K compatible. Peter
  12. Rh-fan
    This is an animation I use in my lessons. It demonstrates the abs/elu methode for detecting a smal amount of antigen. Feel free to view and use it. Peter
  13. Rh-fan
    Depends on the answer you are looking for. The 'ALBA' kit is very good in making the differance between an Rh variant and a weak D antigen (all 12 Monoclonal anti D's will be positive). If one or more monoclonals are negative you are dealing with a variant. The kit is not very good in naming the variant. So I think it is a very good kit for hospitals to determine if you are dealing with a weak D or a variant D. Peter
  14. Rh-fan
    For destroing Kell you need 0,2M. a typing error I think We have the same problem. We use the powder to make 0,01 and 0,2. The 0,01 is fine but the 0,2 is not destroing the Kell antigen completely. It is going from 3+ tot 1+ but not completely gone. We use a strong anti k reagent to test but it is nog working as we wanted. We have planed tot test also other antigens (other Kell and other systems) tot see how that goes. Peter
  15. Rh-fan
    In the dutch guide lines it is allowed to have a antibody in the donor plasma if the titer is below 32. A weak antibody is (as Malcolm stated) no problem, so you do not have to perform the most sensitive methode. Peter
  16. Rh-fan

    p Value?

    Rh-fan replied to janet's topic in Education / Quality

    a late reaction but I missed yours. I agree. But when you are dealing with 2 or more common antibodies it is helpfull. You can change hole the statistics by saing you need 2 (or 3) single antigen reactive cells. In the complex cases you and we are dealing with (like the nice anti Hro you mention) we mostly do special technics like absorption/elutio to determine the specificity. Is you anti Hro patient -D- or some rare african allel?
  17. Rh-fan
    The website is no longer under IBGRL but now under ISBT, on this site LAN, JR and FORS are added. I hope that Malcolm has not send an angry email Peter http://www.isbtweb.org/working-parties/red-cell-immunogenetics-and-blood-group-terminology/blood-group-terminology/
  18. Rh-fan
    They are both healty and the patient (pregnant) has a normal expression of Do (execpt of Hy but she is hetrozygous for it), no further sings of PNH. We have disided to use the original name "Emma" (also the name of my doughter).
  19. Rh-fan
    Talking about Zebra's, have I mentioned the anti Emm that Bristol found in one of our patients. We have a compatibel sister also. The only problem I have with this result is that when you talk about it it sounds the same as "anti M". Then people look at you and say "Why have we send an anti M to Bristol?"
  20. Rh-fan
    The programs Antibodycheck and Rowny antigen plus sounds very usefull. PS IS there realy snow falling at this moment on this website? Peter
  21. Rh-fan
    I have tried one of the first versions (almost 10 years ago) and was not very pleased with it. Maybe it is changed, but I do not know. I am not a big fan of these computer programs, because it is the job of the MT to do the thinking and antibody identification is more then pos/neg (mostly gray, rule 5 of the golden rules). A computer program can never do the same as an experianced MT. This is from a reference lab point of view. When you work in a smaller lab with a lot of generalists it can be a good adition. One problem with this (and other programs that do the same) is that they sometimes give very rare solutions beside the first (very common) solution. That can make some MT's troubeld. Peter
  22. Rh-fan

    p Value?

    Rh-fan replied to janet's topic in Education / Quality

    Malcolm, You are right about the C/D/G antibodies, the p-value will not help you. But that is no reason to not use it with other antibodies, then a low p-value will give you more sertenty about the specificity. Peter
  23. Rh-fan

    p Value?

    Rh-fan replied to janet's topic in Education / Quality

    p value or fisher exact is not done for the numbers but as Phil stated, 1 reactive cell is not enough. Not only for the p value but also for your one feeling of donig the right thing. Peter
  24. Rh-fan

    p Value?

    Rh-fan replied to janet's topic in Education / Quality

    Sounds like Not looking at the fisher exact is like doing an ABO typing without the reverse typing because sometimes there is a reaction we do not want to see. Bending the rules because it is easier. Specialy for reference labs the bar must be higher than for other labs.
  25. Rh-fan

    p Value?

    Rh-fan replied to janet's topic in Education / Quality

    3 to 3 = 0,05 2 to 2 = 0.16 For AABB ok, but gives me a strange feeling (not good)
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