Hello all, The case A 68 years male with a previous record(our hospital's) of A positive clinical data-NHL is undergoing regular transfusion.All of a sudden in our ortho biovue cassettes he showed reaction(2+) with reverse A1 cells.But on running parrallel with immucor method there was no discripancy. On the otherhand we performed the blood grouping with the same sample by Grifols method.shockingly in this method we got 2+ reaction in forward B and the forward typing gave as AB and the reverse typing showed a result as O. [TABLE=class: MsoTableGrid] [TR] [TD=bgcolor: transparent]Method [/TD] [TD=bgcolor: transparent]Anti-A [/TD] [TD=width: 62, bgcolor: transparent]Anti-B [/TD] [TD=width: 62, bgcolor: transparent]Anti-AB [/TD] [TD=width: 60, bgcolor: transparent]A1 cells [/TD] [TD=width: 60, bgcolor: transparent]A2 cells [/TD] [TD=width: 65, bgcolor: transparent]Bcells [/TD] [TD=width: 62, bgcolor: transparent]Anti-D [/TD] [TD=width: 83, bgcolor: transparent]Rh/control [/TD] [/TR] [TR] [TD=width: 73, bgcolor: transparent]Ortho [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]0 [/TD] [TD=width: 62, bgcolor: transparent]- [/TD] [TD=width: 60, bgcolor: transparent]2+ [/TD] [TD=width: 60, bgcolor: transparent]- [/TD] [TD=width: 65, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 83, bgcolor: transparent]0 [/TD] [/TR] [TR] [TD=width: 73, bgcolor: transparent]Immucor [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]0 [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 60, bgcolor: transparent]0 [/TD] [TD=width: 60, bgcolor: transparent]0 [/TD] [TD=width: 65, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 83, bgcolor: transparent]0 [/TD] [/TR] [TR] [TD=width: 73, bgcolor: transparent]Grifols [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]2+ [/TD] [TD=width: 62, bgcolor: transparent]- [/TD] [TD=width: 60, bgcolor: transparent]4+ [/TD] [TD=width: 60, bgcolor: transparent]- [/TD] [TD=width: 65, bgcolor: transparent]4+ [/TD] [TD=width: 62, bgcolor: transparent]4+ [/TD] [TD=width: 83, bgcolor: transparent]0 [/TD] [/TR] [/TABLE] In all the methods the antibody screen was negative.How can we sort this out?please shed light.