khalidm3
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Everything posted by khalidm3
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Always when ever there is an ABO discrepancy, patient history, (age, diagnosis, medication, historical blood group, ABS, blood transfusion etc) should be considered and Lab work should also be performed as described in this case by JOANBALONE. Attempt should be done to solve before the transfusion (shifting to O Cells) unless patient requires it on stat/urgent basis.
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Thanks Received the charts today. Fantastic work. I shall hang on Saturday after lamination. Thanks to CSL Biotherapies and PathLabTAlk.
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There is no reason not to issue DCT + as Malcolm stated but we are not issuing. We are on ABSC and AHG cross match so we get this unit incompatible in coombs and discard it.
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We are testing 200-300 specimen daily for ABO/Rh,ABS, (IAGT/AGAT) and observed nothing like that by DiaMed ID cards.
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C3/C4 Coating http://www.scribd.com/doc/48967246/Compliment-Coating
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... ditto... asI I have an old edition of ARC manual, and Judd's new, so I am using Judds.
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How to upload file in PathLabTalk thread or Library? Can u explain me. I shall wait for ur reply otherwise I shall post somewhere else. If u have this book, it is there. Judd’s Methods in Immunohematology 3rd edition, Published by AABB. Sorry these days I am busy, so less time for our Forum.
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Thanks Anna.
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http://www.scribd.com/doc/48315936/Preparing-IgG-Coated-RBCs
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Please if any one using Ocea Soft (COBALT); how efficient is the system?
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I did not success to up load (Attach the file) Please explain.
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Thanks All our transfusion Lab work is on DiaMed Gel. DiaMed provides Modified LISS solution for preparation of cells suspensions so we prepare our donor cell suspensions in LISS (We perform AHG antibody three cell Screen and AHG cross match for red cell all transfusions). In the leaflet they mention it can be used for cell preparation in the laboratory and the cell will be stable at 2-8 C for one day. They mention also " ID Diluent 2 is a low ionic solution made for DiaMed ID sysytem". I want to use these cell suspension for Immediate spin cross match and repeat blood group checking by tube method from these already prepared suspensions for DiaMed Cards.
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Is it permissible to perform IS cross match, ABO or Rh Group in modified LISS solution?
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AABB Standard 5.1.8.1.2 For storage of blood products, the temperature shall be continuously monitored or the temperature shall be recorded at least every 4 hours. If u have a central monitoring, recording and some one viewing it, Chart may be not required. If monitoring and recording is individual ref to ref; daily once temperature should be recorded on a temperature chart. It is required by our accreditation agency. How AABB/CAP explains it, I want to know also.
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still using albumin for IAT
khalidm3 replied to sheafive's topic in Immunohematology Reference Laboratories
Thanks Malcolm -
still using albumin for IAT
khalidm3 replied to sheafive's topic in Immunohematology Reference Laboratories
Sure LISS enhances better than Albumin, but Albumin does not give false positive/stronger positive like PEG or missed/weaker reaction(Kid, E or K) like LISS We are on LISS by DiaMed now -
still using albumin for IAT
khalidm3 replied to sheafive's topic in Immunohematology Reference Laboratories
The Function of albumin in IAT (Manual tube method) is to enhance the reaction/reduce incubation time. If we do not use Albumin we have to incubate our test for 30-60 minutes and addition of Albumin 22% reduces it to 15-30 minutes. For routine procedure it should be one hour without Albumin and 30 minute by albumin. In stat cases we can reduces it accordingly in the limit. -
Thanks Malcolm, Kind of u, excellent advices from u for all of us who are working in less facilitated and less educated environment. For any confusion my medical director asks me to post in BloodBankTalk and he is reading the forum with quite good interest. I am thinking to use plasma from a B blood donor.
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Thanks, nice to read from China, ur English is good and understandable. All of us benefit from ur knowledge and experience.
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Thanks
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... ditto....
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It is not sub group of B, may be a sub group of A, so the patient may be AB, due to subgroup of A??
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Thanks all of u, the reaction with A1 cells is 3+ by Gel and Non A1 Cells negative till AHG. We will do absorption/elution as Malcolm suggested in his first post. I did not incubate at 4, as I had no more serum while patient was from OPD. I have cells so I shall attempt Malcolm opinion.
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Thanks
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Thanks, Not yet working tech shifted to an other bench. May be day after tomorrow we will continue.