I was wondering what other labs are doing in these situations where you are using Gel>PEG>LISS. Do you run a DAT or Auto control, or both? And if you are switching methodologies, do you run the Auto control for each methodology (ie, run it in Gel, then PEG, then LISS)? We used to just run a DAT, and if we went through all our capabilities to ID an antibody and were unclear or uncertain, we'd send it to our reference lab. Now our supervisor wants us to add the Auto control to EACH antibody identification (whether it's a "simple" ID, or an ID where we need to move to another methodology). I can maybe see where in this may be helpful in the case where we are using multiple methodologies for antibody ID, but don't necessarily see the benefit on doing an DAT and Auto on a "simple" antibody ID. Maybe someone can enlighten me. Thanks!