Maybe we can use CCdee cells to absorb the antibodies in the patient's serum, and then do elution testing . If the anti-E can absorbed by the antigen neg cells , then it is mimiking antibody.
I think we can give Rh positive platelets in this kind of situation, because the recipient's own Rh pos red blood cells is in the circulation now and the Rh antigen in apheresis plts(on the contaminated rbc) is little.
NedB, nice to meet you! You said you will add two drops of saline and re-spin the tube to exclude rouleaux. I don't know if do this will diluet the original antibodies .
I think the problem to use poly and anti-IgG only is the component of poly and the concentration difference. Dose it only contain anti-component and anti-IgG? What is the influence of the concentration difference to the result? Sorry, I have not read all the posts before me because the time limit, if my post have some wrong, please point it out. Thanks!
I have not opportunity to read this article,what a pity! I have some questions, need some help, thanks advance: Is it IgM antibodies caused HDFN? Had the mother experience any invaded treatment, like intrauterine
injection or trauma?
Rh23 is a low frequent antigen expressed in some weak D type blood. In an assay I read it expressed in CCDEE phenotype, but this is not a kind of weak D. What is the name anti-Dw( anti-Rh23) comes from?
I think we may take drug induced hemolysis into account.
There is a link http://www3.interscience.wiley.com/journal/71009042/abstract
If the patient had been found is DAT positive before transfusion, maybe treat it earlier . If duration between the T&S and the transfusion is too long, during this period the patient receive some drug, the antibodies status maybe change.
I have some questions, would some friends kindly help me to resolve it.The patient is D pos,would give him or her Win-Rho destroy his own red cells? What is the purpose of this therapy?
Sorry, my English is not so good, if my understanding is wrong, I beg your pardon. The least incompatible is the least agglutination. I can't understand what is the same of the little pregnant as it, can you tell me?
Some doctor think to transfuse AIHA patients with washed cells because washing can remove allo- protein or something like this, those things can activate the recipients immunization, the factor will let the auto-immune gone worse. Who can tell me whether it is necessary to choise the least incompatible unit for AIHA patients? Thanks advance!
Thanks for your posts. For this kind of patients we usually give the least incompatible units to them. The first unit is the least incompatible one, but the result is not good; the second one is a random one, the Hb is risen. So I think the most important is not the crossmatch but the steroids her been used. The Hb risen is the result of weakened hemolysis.
The doctor insist to transfusion, the patient been given 1 Unit 3 washed red blood cells. After transfusion ,her Hb from 39g/l risen to 55g/l. Although the blood is uncompatible. So I have a question: Is it significant to do crossmatch for the AIHA patients?
We meet a patient, her diagnosis is AIHA. We can't get the specificity of her autoantibodies.Fortunately she have not alloantibodies. Yeaterday we give 1 unit of 3 washed cells to her(not compatible), the Hb fallen . Today the doctor want 1 unit again, what can we do?
To grade the mix field we can differentiate A3/B3 to Aend/Bend subgroup, and when a type A patient been given type O cells ,if not too much,we can see the anti-A agglutinate lots of cells instead of a few of cells which will reflect through the grading report.
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