Yanxia

Velliot also can detect the Fya antigen after transfusion specimen, if there is negtive, the transfused cells destroyed because immune hemolysis caused by anti- Fya and maybe other antibodies we don't know at this stage . If the result is mix-field, and as you mentioned before the post transfusion DAT is neg, I don't think it is immune hemolysis.

I think if she had not transfused it is safe to give tube compatible units.

<removed by admin> Sorry Yanxia, the post you referenced was spam and has been deleted. - Site admin I am very interesting in your post , but I just know Chinese and English.

You say the plasma was icteric, I think the hemolysis can destroy the infused cells in such a short time was intravascular hemolysis, the plasma is hemolyzed not icteric. What about the Fya antigen on the infused cells? It just my guess that the post HGB result, does it drawn during the dialysis, does it consider the dilution during dialysis?

To Malcolm, I think it is interesting that no B transferase gene but have anti-B adsorbtion and elution from the cells. You haven't ask this question to the molecular expert who do this, I remember there is s case of japanese no Aor B transferase gene but express it, it is because gene exchange, maybe, I can't remember how to say it.

QUESTION: What is the cause of lea+b+?

And anti-HNA.

Malcolm, I am very respect of you in this field. But in this question, I disagree with you unless there are some date support you . I am sorry!

IgG have the function of immunosuppression, If my memory don't treat me, it is when IgG Fab bind antigen and Fc bind B cells receptor , this can induce the immunosuppression to this kind of antigen.

But if we give this patient type 0 plasma, the anti-B with bind the B substance in his plasma and the immune complex can bind the receptor on immune cells, this can reduce the recepient's immune ability. Or the IC sedimentation cause hypersensitivity. This is just my guess, it is maybe not true.

I mean plasma seperated from whole blood long than 6 hours after drow。

What is the differnce between common plasma(plasma not fresh) with FP24 ? As to TTP, we will use cryopoor.

Please help me, what is FP24? Thanks in advance!

And antibodies in the anti-A reagent to red cells low frequency antigen. The first one we can wash the cells to avoid it, the second one we can test the cells with AB serum to detect the secret antigen exposue, in the third one, I think it maybe change to other reagent.

Does the cells washed before testing? If not , there maybe anti-prenservation reagent antibodies in the plasma give the false postive result. And if my memeory don't treat me, ther is another posipility is Tn active like A antigen.

Thank you. Our reference lab have do the AHG reactivity , but they can't get anything . This patient compatible with AB cells in AHG phese. As to the 4 degree c and enzyme reaction, I am sorry , I have not do this . And I think this patient have transfused , what will we get from the post transfused specimen, anti-A, anti-B , this can bond on the transfused cells.

Yes , I susperct he is O mistyped with AB and had been transfuised with AB cells. If the patient has shown autoagglutination in the first time , and he is 72 years old ( sorry, I forget to mention it), antibodies is weaker even in healthy elderly people and he is immuno-deficient because disease. I have heard 2 or 3 case of ABO type transfusion error , one is 16 years old , they have not show any sign of hemolysis. It is stranger but it exist. And I agree with your guess, he maybe transfused with O cells and family member's memory is not right.

I noticed you mention they are elderly people, we think people elderly than 60 years old can get weaker reverse reaction, it is common.

I agree with Malcolm.

I think this donor maybe Bx type, because anti-AB result is stronger than anti-A. As to the anti-H result, I wonder what the other cells reaction(2 group B cells , 2 group O cells, we will use those cells as control , in case the reagent is inactive or other case can make the test not exactly). For the reverse type , I think you can put it under 4 degree C 20 minutes to see if you can get some different result to room temperature.

I have try to seperate cells with anti-E and anti-C which all show mixed field reaction . The procedure is add anti-serum (anti-E or anti-C) and patient's cells, centrifuge it and then seperate the agglutinated cells with free cells. Add the free cells to two tube then add anti-A and anti-B respecitvely, centrifuge them , then see the result. I think this can tell me the mix field if comes from two seperatable cells population. It can't differ mosaic with transfusion error. Then I can do saliva substance to detect the A &B substance, if it exist , the patient maybe mosaic, if not then he is transfusion error. But I don't know more about mosaic type, whether they produce A &B substance. Would your friends help me with mosaic type knowledge? Thanks in advance!

I think this is a good way. Saliva and genotyping can't differ ABO subtype with transfusion error and antigen drcrease, I think.

I have a patient is suspect to have suffered MDS. His blood type result is anti-A 2+mf; anti-B 2+s mf, anti-D 3+, Ac neg, Bc neg He has been transfused 7days before with more than 4 units of AB group red cells . I can't get the pre-transfusion specimen, and I suspect they have typed him wrong. and I can't eleminate he is antigen decrease. How can I get the right blood group result? Except waiting...

Thanks, Malcolm. I know it and I am a person who are difficut to persuade,too.ha ha

Antigen 132, would you share your test procedure with us, how can you get this conclsion?