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Yanxia

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Everything posted by Yanxia

  1. Yanxia
    Does the cells washed before testing? If not , there maybe anti-prenservation reagent antibodies in the plasma give the false postive result. And if my memeory don't treat me, ther is another posipility is Tn active like A antigen.
  2. Yanxia
    Thank you. Our reference lab have do the AHG reactivity , but they can't get anything . This patient compatible with AB cells in AHG phese. As to the 4 degree c and enzyme reaction, I am sorry , I have not do this . And I think this patient have transfused , what will we get from the post transfused specimen, anti-A, anti-B , this can bond on the transfused cells.
  3. Yanxia
    Yes , I susperct he is O mistyped with AB and had been transfuised with AB cells. If the patient has shown autoagglutination in the first time , and he is 72 years old ( sorry, I forget to mention it), antibodies is weaker even in healthy elderly people and he is immuno-deficient because disease. I have heard 2 or 3 case of ABO type transfusion error , one is 16 years old , they have not show any sign of hemolysis. It is stranger but it exist. And I agree with your guess, he maybe transfused with O cells and family member's memory is not right.
  4. Yanxia
    I noticed you mention they are elderly people, we think people elderly than 60 years old can get weaker reverse reaction, it is common.
  5. Yanxia
    I agree with Malcolm.
  6. Yanxia
    I think this donor maybe Bx type, because anti-AB result is stronger than anti-A. As to the anti-H result, I wonder what the other cells reaction(2 group B cells , 2 group O cells, we will use those cells as control , in case the reagent is inactive or other case can make the test not exactly). For the reverse type , I think you can put it under 4 degree C 20 minutes to see if you can get some different result to room temperature.
  7. Yanxia
    I have try to seperate cells with anti-E and anti-C which all show mixed field reaction . The procedure is add anti-serum (anti-E or anti-C) and patient's cells, centrifuge it and then seperate the agglutinated cells with free cells. Add the free cells to two tube then add anti-A and anti-B respecitvely, centrifuge them , then see the result. I think this can tell me the mix field if comes from two seperatable cells population. It can't differ mosaic with transfusion error. Then I can do saliva substance to detect the A &B substance, if it exist , the patient maybe mosaic, if not then he is transfusion error. But I don't know more about mosaic type, whether they produce A &B substance. Would your friends help me with mosaic type knowledge? Thanks in advance!
  8. Yanxia
    I think this is a good way. Saliva and genotyping can't differ ABO subtype with transfusion error and antigen drcrease, I think.
  9. Yanxia
    I have a patient is suspect to have suffered MDS. His blood type result is anti-A 2+mf; anti-B 2+s mf, anti-D 3+, Ac neg, Bc neg He has been transfused 7days before with more than 4 units of AB group red cells . I can't get the pre-transfusion specimen, and I suspect they have typed him wrong. and I can't eleminate he is antigen decrease. How can I get the right blood group result? Except waiting...
  10. Yanxia
    Thanks, Malcolm. I know it and I am a person who are difficut to persuade,too.ha ha
  11. Yanxia
    Antigen 132, would you share your test procedure with us, how can you get this conclsion?
  12. Yanxia
    dosage effect?
  13. Yanxia
    Thanks Malcolm, I have learn lot things from your post. There is a question troubled me, can you tell me why we test the anti-A use A1&A2 cells both.
  14. Yanxia
    Thanks Anna. I suspect it is antigen weaken by disease. Some cells' H antigen is not so strong expression so give this kind of result. But I can't do anything except waiting her recovery.
  15. Yanxia
    Why ? Because the reagent or the antigens? Oh, she has not received any transplant.
  16. Yanxia
    I encountered a patient, her diagnose is AL. She has not received any red cell components. Her ABO type is anti-A 2+ mix field, anti-B neg, anti-A1 neg, anti-AB 2+ mix field, anti-H 3+mix field is stroger than B cells and weaker than O cells , her serum is neg with A1cells and 3+with B cells. anti-D, anti-C,anti-c,anti-E,anti-e all 3+ without mix field. I am very interesting in the H antigen result, why it is mix field, and what can I do next to get the answer, please help me. Thanks!
  17. Yanxia
    Maybe the DAT pos cells all hemolyzed , at least below our test sensitivity. After 3 days to retest the antibody screen maybe we can get something significance.
  18. Yanxia
    Why to remove it?
  19. Yanxia
    Thank you, Fluffy. I want to know if the anti-B reactive in 37 degree C. And thank you, Malcolm. Can you explain why this lady transfuse group O cells stimulate an anti-H would not cause a big problem with another pregnancy, dose anti-H can't cause HDFN? Thank you again.
  20. Yanxia
    Me too,thanks.
  21. Yanxia
    Transplant is a cause of discrepancy, but I have a double about wether this kind of people can get pregnant.
  22. Yanxia
    I will try 1. A subgroup B 2..congenital anti-B deficient 3. potent anti-B I just can remember this two explanation. The further investigation is to extension the incubation time with human anti-B in 4 degree C and/ or adhesion and elution test to the B antigen. To test the saliva Blood group substances. To prove the second explanation is to do the immunoglobulin Quantitative. 3. delute the serum to avoid Prozone phenomenon
  23. Yanxia
    Or the donor's T antigen exposure.
  24. Yanxia
    Maybe it is something make the cells rouleux to give fail positive result in gel. To see the cells under microscope maybe give you some help.
  25. Yanxia
    In small lab it is harder to differ the allo and auto anti-e like this case. I don't think there is any mean to know whether it is allo or auto anti-e in blood transfusion. Beacause we need give him e neg blood in two case.
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