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Everything posted by javvcr
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Hello! In our lab, we are studying the implementation for MMA Is someone running this technique? Which protocol do you use? Which "recipe" are you using? Thanks!
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Hello everybody! Can you tell me, under which indications, do you use wash RBC´s for transfusion? Greetings to all!
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In our facility we just started to use this typing, antibody screening and crossmatch system, we´d love to read some about your experience! Thanks!
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Hello everybody! i'll like to know if somebody uses infrared thermometers for donor tempeture measure? if someone does, what is your experience, do u like it, donors like it? Please let me know!!!
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Hello everybody! I have a question to all of you! Do you deferral the people who come to donate who have the disease called vitiligo, and if you do, the deferral is permanent or temporary?
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Hello! I would like to know, if someone is certificated and to wich standard: ISO, AABB, FDA or something else???
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hello everybody! these days, there came a blood donor who consumed Xenadrine. any of you know or suspect a contraindication to donation of blood from people taking fat burners?
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False Positives for HTLV Abs and HBc Abs?
javvcr replied to Liz's topic in Donor Services and Donor Recruitment
we in Costa Rica, performe HTLV in microplates. Murex 4 years ago, and Diasorin, now at days. because, HTLV, has a very low prevalence in our population, so many false positives are found. i can tell that 1-2% of our donors test positive for HTLV. We repeate it two times more from pilot tube and two form the bag. (previosly centrifugation at 3000 g) if it continuos positive, we perform a westernblot for HTLV. mostly are negative. -
Auto control and DAT negative!
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hello everybody! i would like to know, how do you evaluate an antibody that reacts with I, II and III screening cells, no especifity determinated with panel (all positive 2+)? this case, doesn´t present a history of transfusion or pregnacy. he is a candidate for renal transplantation donor. what is your regular work flow in this cases???
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Probe, DAT on the sample of the red blood cell transfused, some times, blood donors appears with DAT positive. Try also, if you want, washing the red cells, before elution, with, cold LISS solution, in case you DAT is positivo for low avidity antibodies! Did u tested DAT in monospecific reactives (anti IgG and anti C3?), if you have a DAT positive with polispecific reagent, so you most look for the monospecificity
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Hello! this is my doubt. We are using Axsym for blood donor screening, and we have some anti core (total) positives (low value). after that, we rescreen the blood sample in architect, and it give us a negative value. does someone have any experience like that at your services?? does someone have any scientific (really scientific) explanation ???? thanks!
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Thank you Malcolm! I have another question!, Using Donor Grouping card (Diamed´s), they come with a reactive for D, that detects D(VI+). so when u have a negative reaction, do u confirm it with convetional protocols for negative D in donor, or you just asume it´s a real negative, reporting and labeling the RBC as Rh negative?
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is someone using SS at their blood bank or transfusion service? how have been your experience? good and no good things about it!
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Is out there, any one, using Diamed gel?? is it FDA approved???
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Hello! i'll like to know, what technology are you using for blood typing, red cell antibody screening and crossmatching?? the good, the not that good, and the bad about them! thanks!
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this is the reason for my question! in case you have a patient with an antibody identificated, and need a transfusion today. But later on (a week or a month later) he or she needs another transfusion: Do you performe the antibody screening and full identification again, then give blood antigen negative, cross match negative or performe the antibody screening and cross match and just if the cross match appears positive, you do the antibody identification again? or do you do something else????
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hello everybody! here is a question killing me this days! what do you do when a M, N, P1, Le (a), Le ( or Lu (a) does appear in the antibody screening before transfusion requierment?
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Could somebody help me out with this? i'll like to know what is the difference (if it does exist) between these test or is that those tests give the same information?? :mad:
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i guess diamed, has those lectins too! ask our supplier!
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this is a consultation, could someone, tell me what type of methods do you performe, for investigation of rare Rh antigens? antisera, panels , etc!
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i have to ask you if someone have ever been in this situation berofe! in our lab, we used to performe our panel control weekly, using a commercial anti Jka (IAT use). but in this occation, with a new lot, and a new supplier, our Panel (Diamed), give us a nice identification, but our Panel E (papain treated, and Diamed too), give us all the eleven cells, negative. I was so concern but, i decide to use bromeline treated Cell (surse from the untreated panel) and the reaction were enhanced like i spected. Resuming, our Panel E, enhance perfectly, all the others antibodies, but, this one, this particular supplier, didn't. have someone ever have a situation like that before! jimmy
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hello everybody! i'll like to ask you all, that mesures have been taken in your facilities, to deal with this new tipe of influenza! thanks! javvcr, from Costa Rica!
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Yeah! i think its kinda difficult but i guess our criteria of permanent deferral should prevail. It just a in vouge issue in our country from people out of the medical area, so medical criteria should be follow. any way, in countries like Spain and Japan, MSM have a 12 month deferral and are accepted like donors!!!!!! Have u heard something about it?
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In our country, we use to deferral permanently male who have had sex with other males! but this has been cause of a very interesting discution 'cos here the *** movement is so strong, and they want to change this policy, 'cos they feel its discriminatory for them! tnx