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May 2024 Challenge

marilynm

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Everything posted by marilynm

  1. marilynm
    Commercial Anti-A1 lectin has been diluted so that it will only react with A1 cells. Adding it to human plasma will definitely dilute it, but it is pretty strong in reactivity so it should be able to be diluted quite a bit. I think the question is "why it gets weaker after a day or so after being added to human plasma?". when you mix monoclonal antibodies with human antibodies of the same specificity (Rh D for instance), you sometimes get a weakening of the human antibody. Perhaps the human ABO isoagglutinins are affecting the Anti-A1 lectin (prepared from seeds). I will ask my manufacturing experts and get back to you! Marilyn PS I too have to consult experts for some questions! Sorry to be so longwinded, which is my character, as you know. You may be sorry that I am back!
  2. marilynm
    EGA is available commercially as a kit, which has a good experiation date and can be used in other situations for removing IgG for typing RBCs for other antigens, or there are procedures for making up the solutions and validating them. I understand that small laboratories may not be able to keep the kit or solutions, but most reference labs have them. Marilyn M
  3. marilynm
    If you can find a group A2B from your employees, a blood supplier's donors, etc, then you could just use anti-A1 lectin without adding it to anything. I know A2B samples might be hard to find, but if you do find one, you can keep it for a long time if you store the cells in a preservative solution. mmoulds
  4. marilynm
    I think Mabel Adams is doing great serology. whenever possible, determine the Correct Rh type of these babies so there is no confusion. Just be sure you follow the package insert for the test and/or validate the test if outside the package insert's recommendation for use. EGA is used to strip IgG from the RBCs to facilitate typing by the IAT when the RBCs have a negative DAT. Marilyn Moulds, Immunohematologist, Lecturer and Educator
  5. marilynm
    Just want to say hi to all those names I recognize. Do not know how often I will check this site, now that I am retired, but will enjoy reading the postings. Marilyn Moulds
  6. marilynm
    I forgot to ask if the patient is female and has a history of any pregnancies? We had a White pregnant female who had alloanti-C in her serum, was C-positive. There have also been examples of naturally occurring anti-C. marilynm
  7. marilynm
    Alloanti-C in C-positive people have been reported several times in the literature. Check the Antigen Facts book of Reid and Lomas-Francis. If the person is Black, then their red cells could be C+weak and VS+, and this is the type of situation that most often occurs when there is alloanti-C in C positive people. Is the person Black? marilynm
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