jbeauvais

It doesn't make any difference in the treatment of the patient, except that it was originally typed as a Group O and sent to us because the forward and reverse didn't match. I also thought that since the B subgroups are "rare" (at least here) someone might be interested in the specimen.

Thanks Malcolm

Does anyone know of someone researching ABO DNA? We have a pregnant woman who appears to be a B3 Rh positive. In Gel she forward like an O and reverses as a B. There is a slight haze in the Anti B. By tube using Ortho reagents she types 1-2+ mixed field with Anti B and Anti A,B and reverses as a B. From her name she is probably of Far Eastern (Indian) descent. The only time I have seen a B3 was on my SBB practical, back in the days when they were sending wet samples. I won't say how many years ago that was.

We had a problem with Ortho Cell#2 in lot VS286 the first day it was put into use. A 4+ reaction with it. The tech pulled some cells with low incidence antigens (from another manufacturer) and identified it as Mg. The patient's serum reacted with 2 other examples of Mg+ cells. Jeanne

That was the perfect paper I needed. We were also wondering if anyone in the US uses an IgG cover for patients with multiple antibodies.

We recently had a sickle cell patient with a previous Anti-U hyper hemolyze after transfusion of U negative units. In researching this problem we came across a paper from the UK about using an IVIgG cover to transfue patients. Can anyone explain what this is? Is it using the same theory as giving IVIgG to ITP patients? Thanks Jeanne in Michigan

Does anyone have a method of cleaning the stem cell bag specimen to get free red cells for typing? Most of the time we can get an ABO type but we have had a least two occasions where we can't get the bag specimen to type correctly with RH antisera. We can't get enough cells to type correctly with Anti-D. The original pherpheral blood sample types as D Pos.

I guess I should have tried to be a little more creative with the logon. Can't hide anywhere.

I'm having trouble making examples of Anti-A1 for teaching purposes. I've been making them for years using A2 patients with A1 lectin & pt. serum. Lately it seems that when I make and test the sample it's 4+ right after I make them. The next morning the reactions are negative. Anyone have a recipe for making and keeping an Anti-a1 reactive? Jeanne