diplomatic_scarf

Welcome! You came to the right place! The members here are experts in BB and quite knowledgeable in the field. Good luck with your studies.

It has been over 100 years since the ABO blood types have been discovered, but we still don't really know what are their "function"? What exactly do they do? What did they evolve from? Thank you

I doubt that would be likely, since I think a positive antibody screen disqualifies donors. But maybe I am wrong.

In plasma products, what exactly would "spike" the formation of Anti-D? Residual D-positive RBCs or platelets?

You wouldn't happen to know the Fisher formula or where I can find it? I am just curious about the actual math/statistical tool(s) involved in the rule of 3. Thank you.

Rarely you will find an WAA stronger than an underlying alloantibody. That is what makes this method useful. The weaker autoantibodies won’t react and only the stronger alloantibodies will react. Attached is the AABB sop for this method. METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf

I know this thread is several years old but I just want to say I agree with Malcom. Also, i just want to add that I have been researching this method for the last few days, and found out that many labs use this method. The main idea here is that alloantibodies usually have a stronger reaction that WAAs. So if you don’t use saline without any enhancements, you will only get the alloantibodies reacting. This especially works well when the underlying alloantibodies are 2+ or greater and the WAAs are 1+ when done with enhancement. It’s a “quicker” way to identify alloantibodies without doing an autoadsorption.

Thanks everyone for your input! I found the method on the AABB website. It's attached if anyone wants to see it. Thanks again! METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf

This the tube method? Thanks so much ! How many tubes do you use for your panel ?

Thank you for your response. What is the ingredient in this saline? Do you know the manufacturer name or the procedure steps? Thank you.

Thank you for your post. I am sorry, but what is NSF? Do you know the name of the manufacturer of this saline panel? or know of any sources about saline IgG? Thank you for your time and help.

Anyone here has any experience with saline IgG and it's use in detecting underlying alloantibodies? In a situation that may involve possible multiple alloantibodies or warm autoantibody(ies). I would like to find out more about the principle and the procedure behind this. Thank you.

Yes, it is very helpful! Thank you Malcolm.

Thank you Malcolm. Would you have any sources/web sites on this subject? I would like to read more on the subject if possible. I tried googling it but with no success. Thank you so much!

What is ‘+s’? Please look at attached antigram. It’s in the reaction column of the P1 antibody. Anybody knows? Thank you

"Sure it could happen ,,, and here's how... one of the parents perhaps has the Bombay phenotype"

I disagree. Most gel cards and Anti-D reagents won't detect DVI for patients. Fortunately I can find numerous suitable quotes, because it's true. https://labs-inc.org/pdf/361_3.pdf

Yes, I know. I was talking about patients on my original post.

"Modern anti-D reagents, while they are very good at detecting weaker forms of the D antigen, are specifically designed to NOT detect the most common form of partial D in Caucasians (DVI, or “D six”), so most Caucasian partial D patients will test as D-negative." -BloodBank Guy https://www.bbguy.org/education/glossary/glp04/

I think most modern Anti-D reagents won't detect DVI and these patients will test as D-negative. This is probably the answer. Anyways, this is the answer he gave his students. To me, the answer looks as vague as the question. Not "straight forward" at all. IMG_2756.heic

Yes, I don't think the original question setter meant the question to be a difficult one to answer. He is teaching a beginner level MLT course. He said there was only one correct "straight forward" answer. Just my opinion , but I think this person has no business teaching college level blood banking. As far as I know, he is a MLT with no experience with the tube, slide, or microplate testing methods, so I highly doubt he was talking about Anti-D reagents being the source of the discrepancy. But I could be wrong. I apologize for wasting people's time with this. I just can't understand how is he teaching medical lab science and blood banking at a local college, and handing out assignments with these sort of questions.

No DAT. There are no other details to the question. Also DATs are not required testing for blood donations. I’m just trying to get people’s opinion on the question. Personally I think it is a poorly written question and the person who wrote it has no clue what he’s talking about, but I’m here to get people’s thoughts on it. Thank you

"A donor unit obtained from a central blood bank was labeled as Group O, D-negative. When the hospital transfusion service confirmed the donor's type, the result was group O, D-positive. Investigation of the label issued at the blood bank verified the unit's correct labeling. How can you explain the discrepancy in the D type of this donor unit?" The person who wrote this question said it is a "critical thinking" question and there is only one correct answer.

I think this question is suited more for sysmex IT tech support. I don't know about these days with modern day techs, but in my experience, it's the techs that perform the cell counts using a miller disc for the KB method. If your facility uses 300 uL dose RhIg, you can use this simple equation: (%fetal cells x 50) / 30 = number of vials of RhIg required. Remember to use the "fudge factor". Example: 1.3% fetal cells calculated on Kleihauer Betke stain 1.3 X 50 = 65 ml of fetal blood 65 ml/30 = 2.2 vials of RhIG required When the number to the right of the decimal point is less than 5, round down and add one dose. Example: If the calculation comes to 2.2, give 3 vials. When the number to the right of the decimal point is greater than 5, round up and add one dose. Example: If the calculation gives 2.8, give 4 vials. source: https://hsc.ghs.org/obgyn-clinical-practice-guidelines/inpatient-obstetrics/kleihauer-betke-assay-interpretation/

I have never heard of your references. The main texts for my course includes AABB technical manual by Fung, Harmening's Modern blood banking and transfusion services, and AABB's Standards for blood banking. I am only a part time SBB student, I work full time as a Medical Technologist. This is my final semester. You don't need to show me references I never heard of, I am certain you are right. Mom's with PARTIAL D (NOT WEAK D, WE DON'T TEST WEAK D FOR MOMS) can be typed as Rh positive, but still may form Anti-D when exposed to Rh positive red cells from baby(Modern Blood banking and transfusion services, Harmening, 7th Ed. p. 160).