diplomatic_scarf

You wouldn't happen to know the Fisher formula or where I can find it? I am just curious about the actual math/statistical tool(s) involved in the rule of 3. Thank you.

Rarely you will find an WAA stronger than an underlying alloantibody. That is what makes this method useful. The weaker autoantibodies won’t react and only the stronger alloantibodies will react. Attached is the AABB sop for this method. METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf

I know this thread is several years old but I just want to say I agree with Malcom. Also, i just want to add that I have been researching this method for the last few days, and found out that many labs use this method. The main idea here is that alloantibodies usually have a stronger reaction that WAAs. So if you don’t use saline without any enhancements, you will only get the alloantibodies reacting. This especially works well when the underlying alloantibodies are 2+ or greater and the WAAs are 1+ when done with enhancement. It’s a “quicker” way to identify alloantibodies without doing an autoadsorption.

Thanks everyone for your input! I found the method on the AABB website. It's attached if anyone wants to see it. Thanks again! METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf

This the tube method? Thanks so much ! How many tubes do you use for your panel ?

Thank you for your response. What is the ingredient in this saline? Do you know the manufacturer name or the procedure steps? Thank you.

Thank you for your post. I am sorry, but what is NSF? Do you know the name of the manufacturer of this saline panel? or know of any sources about saline IgG? Thank you for your time and help.

Anyone here has any experience with saline IgG and it's use in detecting underlying alloantibodies? In a situation that may involve possible multiple alloantibodies or warm autoantibody(ies). I would like to find out more about the principle and the procedure behind this. Thank you.

Yes, it is very helpful! Thank you Malcolm.

Thank you Malcolm. Would you have any sources/web sites on this subject? I would like to read more on the subject if possible. I tried googling it but with no success. Thank you so much!

What is ‘+s’? Please look at attached antigram. It’s in the reaction column of the P1 antibody. Anybody knows? Thank you

"Sure it could happen ,,, and here's how... one of the parents perhaps has the Bombay phenotype"

I disagree. Most gel cards and Anti-D reagents won't detect DVI for patients. Fortunately I can find numerous suitable quotes, because it's true. https://labs-inc.org/pdf/361_3.pdf

Yes, I know. I was talking about patients on my original post.

"Modern anti-D reagents, while they are very good at detecting weaker forms of the D antigen, are specifically designed to NOT detect the most common form of partial D in Caucasians (DVI, or “D six”), so most Caucasian partial D patients will test as D-negative." -BloodBank Guy https://www.bbguy.org/education/glossary/glp04/