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    I like playing the guitar and singing karaoke.
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    I am a MLS(ASCP)SBB working in a small medical laboratory in America. Ask me anything about blood bank stuff if you like, I will try to answer your questions. I enjoy working in the blood bank.
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    Medical technologist , Specialist in Blood Banking. MLS(ASCP)SBB.

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  1. I think this question is suited more for sysmex IT tech support. I don't know about these days with modern day techs, but in my experience, it's the techs that perform the cell counts using a miller disc for the KB method. If your facility uses 300 uL dose RhIg, you can use this simple equation: (%fetal cells x 50) / 30 = number of vials of RhIg required. Remember to use the "fudge factor". Example: 1.3% fetal cells calculated on Kleihauer Betke stain 1.3 X 50 = 65 ml of fetal blood 65 ml/30 = 2.2 vials of RhIG required When the number to the right of
  2. I have never heard of your references. The main texts for my course includes AABB technical manual by Fung, Harmening's Modern blood banking and transfusion services, and AABB's Standards for blood banking. I am only a part time SBB student, I work full time as a Medical Technologist. This is my final semester. You don't need to show me references I never heard of, I am certain you are right. Mom's with PARTIAL D (NOT WEAK D, WE DON'T TEST WEAK D FOR MOMS) can be typed as Rh positive, but still may form Anti-D when exposed to Rh positive red cells from baby(Modern Blood banking and transfusi
  3. We do not do Weak D testing on mothers here where I work. This is a common practice in America
  4. We don't do Weak D testing on adults, unless if they are donating blood.
  5. I never said mother with weak D. Come on Malcolm, you know I didn't say mothers with weak D.
  6. You know why Malcolm. If mom is Rh negative and baby is Rh positive, mom has 16% chance of developing anti-D after her first pregnancy. Moms with partial D can be classified as Rh Positive, but may still require RhIg to prevent HDFN. Our policy is to do weak D testing on all newborns who test D negative when we do the ABO/Rh forward typing.
  7. We do weak D testing on all cords bloods that test negative with Anti-D. Regardless if the mom is Rh positive or negative.
  8. I am looking for an animation program or drawing program that can help me make my blood bank presentation more entertaining. Anyone knows of such program? This is my last semester in my SBB program and I am required to do a presentation. My topic is on the blocked D phenomenon and it's role in HDFN. I would love to do something like what Dr. Chaffin does for his presentations on his website www.bbguy.org . Any tips or info is greatly appreciated. Thank you.
  9. Yes, you are completely right Malcolm and others. My experience is limited to common positive DAT results for OB patients, which is the ABO mismatch. Those positives are usually 1+ or weaker. That's why we always do a microscopic if we get a macroscopic negative. I forgot that Rh mismatch DATs can go from 2+ to 4+ positive, because I hardly ever come across those. Thank you.
  10. sorry, I shouldn't have said "never" ... What I really meant to say was, I always do a microscopic exam for agglutination if the immediate spin appears negative. Other people probably see a lot of macroscopically positive DATs. I don't come across too many of those. Thank you sir
  11. I think you need to do microscopic on all DATs , because IgG is only a monomer antibody and won't cause agglutination visible to the naked eye.
  12. I am sorry, but what does putting up "12 different antibody screens(12 different patients)" have to do with my original question? Sorry, I don't understand what you are trying to explain. My English is very poor. Can you explain with simple English or use scientific terms instead? But thank you so much for your time and knowledge .
  13. Anyone seen those blood bank guy videos on YouTube, or on his bloodbankguy website? Does anyone knows what does he use to make those blood bank animations? It looks like he is using powerpoint , but he is able to draw cells and antibodies and their reactions in his videos. Does anyone knows what does he use to do that? I wrote him a while back and he hasn't replied. I want to create my own blood bank presentation like that. Thank you for your time.
  14. Thank you so much for your reply. I keep asking you questions, so sorry. But did you say 12 screen cells? Usually, we use only 2 or 3 screen cells. And we have 12 cells in our antibody identification panel. Also , not sure what is "put up". What did you mean by "put up"? You putting something up, like up above something? Sorry, English is my 2nd language, I have a hard time understanding English sometimes. Thank you so much for your time and valuable knowledge.
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