Jennifer G
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Everything posted by Jennifer G
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We are switching to Greiner tubes from BD. We average about 150 RBC transfusions per month. We use Ortho gel for ABSC and ABID and tube testing for everything else with Immucor reagents. What would be considered an adequate number of specimens for a parallel study? Would every test we perform have to be done? Can the number of specimens vary with the type of test? When we rarely use tube testing for ABSC or ABID and when we do comparisons every six months, we have to change the 0.8% cells to 3% which is time consuming. We don't have the staff for exhaustive studies.
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We are switching to Greiner tubes from BD. We are a small transfusion service averaging about 150 RBC transfusions per month. We use Ortho gel for ABSC and ABID and tube testing for everything else with Immucor reagents. What would be considered an adequate number of specimens for a parallel study? Would every test we perform have to be done? Can the number of specimens vary with the type of test? When we rarely use tube testing for ABSC or ABID and when we do comparisons every six months, we have to change the 0.8% cells to 3% which is time consuming. I want to adequately check the Greiner tubes without going insane. SMiller, what did you do? Thanks for your help!
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Thanks for your replies! More info related to your comments... A DAT was not done at the time the antibody was identified, because the auto control in gel was negative. The patient has only received A Negative RBCs at our hospital (total of 9 at that time and 3 since then) and no platelets. She is a nursing home resident that was admitted for pneumonia, respiratory failure, and UTI. She has a previous history of coronary bypass surgery where she may have received blood products. Her platelet counts have always been in normal range 300-400s in our records. The DEL idea seems plausible to me. The Anti-D was relatively weak with reactions ranging from w+ to 2+.
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Has anyone seen an Anti-D go from negative to 2+ positive to negative? We had negative antibody screens on an elderly A Negative woman from 2012 through 2018. 8 RBCs were transfused during this time. In August 2018, the antibody screen was 2+ positive, Anti-D was identified, and she received 1 RBC. In April 2019, the antibody screen was negative on 2 different occasions. The possibility of the August 2018 specimen being the wrong patient seems unlikely since we use hand-labeled separately armbanded specimens. However, I have never seen a true Anti-D behave this way.
