ESIZENSKY 15 Posted December 18, 2018 I am validating DAT's in the gel method (Biorads). I have several DAT's (Cord) positive in tube, confirmed LUI freeze ABO antibodies) negative in gel. Has anyone had this problem when validating in gel. Share this post Link to post Share on other sites
noelrbrown 27 Posted December 20, 2018 Which gel are you using and what is the source difference for the anti IgG i.e. monoclonal versus polyclonal or is it anti complement issue you are experiencing? Share this post Link to post Share on other sites
