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ESIZENSKY

DAT validation in gel

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I am validating DAT's in the  gel method (Biorads). I have several DAT's (Cord) positive in tube, confirmed LUI freeze ABO antibodies) negative in gel.

Has anyone had this problem when validating in gel.

 

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Which gel are you using and what is the source difference for the anti IgG i.e. monoclonal versus polyclonal or is it anti complement issue you are experiencing?

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