Patient comes in with no history. Ortho gel panel A is all positive. DAT in gel is positive. Eluate in gel panel A has two negative cells, gel panel B is all positive with the eluate (strength of reactivities 1-2+). Our policy for the ABID is to send to our reference lab if it is also positive in tube. I feel like it is a WAA just forming bleeding through into ABID. 

How should I proceed with the eluate? Has anyone seen a WAA that has two negative reactions in gel? 

Thanks! 

Yes, not that unusual actually.

Are your cells that is non reactive with eluate either e- or f-? did you have antigen typing on this patient? Was the patient recently transfused? 

Edited June 14, 20187 yr by dothandar