Warm Auto Activated Through Storage

[longhorn2891]

Has anyone encounter the following anomaly?  If yes, please enlighten us with possible causes or references which may provide an explanation.  Thanks!

 

Patient has history of anti-C and anti-Jkb

Antibody screen negative on freshly collected sample.

Compatibility testing with C and Jkb negative units were compatible.

Sample stored in fridge overnight.

 

Sample was pulled out of fridge to perform crossmatch for add-on orders.

All crossmatches were incompatible. Screen repeated and everything was positive. Panagglutination observed on panels.

 

Phenomenon persisted with 3 subsequent samples.

[Malcolm Needs ☆]

I have only heard of this phenomenon once before, when it was described to me when I was lecturing on a course in Scotland.  I had no definitive answer then, and I still have no definitive answer.  I just wonder, however, if the storage in the cold causes some of the molecules in the plasma, such as albumin, to aglomerate, and it is this that causes the phenomenon - but this is only a guess.

[longhorn2891]

I have only heard of this phenomenon once before, when it was described to me when I was lecturing on a course in Scotland.  I had no definitive answer then, and I still have no definitive answer.  I just wonder, however, if the storage in the cold causes some of the molecules in the plasma, such as albumin, to aglomerate, and it is this that causes the phenomenon - but this is only a guess.

 Thank you for the interesting plausible insight.