Posted June 18, 201312 yr comment_51443 Patient is group O in forward typing but the reverse demonstrated no reactions in both A1 cells and B cells. I tried incubating the serum with the reagent red cells at room temperature for 15-30 minutes but still yielding no reactions. I tried another approached by incubating the serum with the reagent red cells at 4o C for 15-30 minutes but only got an M+ on A1 cells but still no reactions in B cells. What do you think is causing this? Thanks!
June 18, 201312 yr comment_51446 I agree. We need more information, such as age, underlying pathology, etc.
June 18, 201312 yr comment_51449 Reverse should be*incubated at 37 C for IgG/IgM dosage effect (or incubated at 4 C for 30 min. you had done),*checked for age Newborn or elder >60 years old,*agammaglobulinemia (check for gamma globulin level by ELISA)*may be using immunesupressive drugs...
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