Anti Fyb or anti Fy3

[Rh-fan]

We have a patient with the Fya-b- phenotype, on DNA she is FYBB and homozygous for the GATA1 mutation. So we can expect an anti Fya.

The serum is reactive with all cells expressing Fya and/or Fyb antigens, and non reactive with Fya-b- cells (beside the ones that are K positive).

We expect an anti Fy3 but in gel this antibody is only reactive in LISS (IAT) but not in Papaine LISS (IAT), therefore more pointing to anti Fyb.

We have never seen an anti Fya + anti Fyb in such a patient.

We are goint ot absorb the serum with cells expressing only Fya and with cells only expressing Fyb and test the absorbed serum and the eluate of the absorption cells, any other suggestions?

Peter

[Malcolm Needs ☆]

I suppose it isn't the first example of a human anti-Fy6?

[Rh-fan]

That would make me very happy. But first we do the absorption experiments to see if it is Fyb or Fy3, maybe then we can do a MAIEA with monoclonals Fy3 and Fy6.

Keep you informed