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Gentle Heat Elution

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when using 6% albumin to obtain a heat eluate, is there any reason we can NOT use PEG when testing it ( to increase reactivity) ??:confused::confused:

jane

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If I was doing that I would validate the procedure - what would you be looking for besides an ABO ab?

we use it mostly to prepare auto cells for autoadsorption and then use the eluate to "prove" the warmauto. we also use it in teaching situations to show eluate options. we have validated it and it works well. just wanted to make sure there wasn't some type of chemical rxn between 6% albumin and PEG that i don't know about.

jane

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